Detailed information of TA system TA185736

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 111488..112260
• Replicon: chromosome
• Accession: NZ_CP067093
• Organism: Klebsiella michiganensis strain B106

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: IMZ21_RS00510
• Protein ID: WP_117042591.1
• Coordinates: 111488..111877 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IMZ21_RS00515
• Protein ID: WP_177903626.1
• Coordinates: 111934..112260 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMZ21_RS00480	107158..107481	+	324	WP_004128377.1	endoribonuclease SymE	-
IMZ21_RS00485	107622..108056	+	435	WP_117076050.1	VOC family protein	-
IMZ21_RS00490	108222..108683	+	462	WP_049113955.1	hypothetical protein	-
IMZ21_RS00495	108717..109448	+	732	Protein_94	hypothetical protein	-
IMZ21_RS00500	109623..110420	-	798	WP_117042594.1	helix-turn-helix transcriptional regulator	-
IMZ21_RS00505	110564..111409	-	846	WP_154909414.1	DUF4942 domain-containing protein	-
IMZ21_RS00510	111488..111877	-	390	WP_117042591.1	TA system toxin CbtA family protein	Toxin
IMZ21_RS00515	111934..112260	-	327	WP_177903626.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IMZ21_RS00520	112318..112539	-	222	WP_117042589.1	DUF987 domain-containing protein	-
IMZ21_RS00525	112553..113032	-	480	WP_047724681.1	DNA repair protein RadC	-
IMZ21_RS00530	113044..113487	-	444	WP_117042588.1	antirestriction protein	-
IMZ21_RS00535	113567..113824	-	258	WP_177903624.1	DUF905 domain-containing protein	-
IMZ21_RS00540	113821..114645	-	825	WP_117042586.1	DUF945 domain-containing protein	-
IMZ21_RS00545	114900..115727	-	828	WP_117042585.1	hypothetical protein	-
IMZ21_RS00550	116355..116945	+	591	WP_200483091.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	107622..145131	37509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14401.60 Da
• Isoelectric Point: 9.9963

>T185736 WP_117042591.1 NZ_CP067093:c111877-111488 [Klebsiella michiganensis]
MQTKSSPLMRAASSRPSPVGVWQTLLACLLEHHYGLTLNDTPFSDELVIQQHIDAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITAIDILRARKATGLMNRGTYKEVTAITRGLHRQVSIPGKR

Nucleotide

• Length: 390 bp

>T185736 NZ_CP089012:c5062459-5062076 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12083.84 Da
• Isoelectric Point: 6.9461

>AT185736 WP_177903626.1 NZ_CP067093:c112260-111934 [Klebsiella michiganensis]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHLVTLYA
KGLTCEADTLGSHGYVCIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT185736 NZ_CP089012:c5062701-5062459 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA