Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20096..20621 Replicon plasmid pSLT
Accession NZ_CP067092
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain ER3625

Toxin (Protein)


Gene name ccdB Uniprot ID I3W3D5
Locus tag JJB80_RS23285 Protein ID WP_001159863.1
Coordinates 20316..20621 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID M7S5D0
Locus tag JJB80_RS23280 Protein ID WP_000813641.1
Coordinates 20096..20314 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB80_RS23255 (JJB80_23250) 15383..15764 + 382 Protein_20 YgiW/YdeI family stress tolerance OB fold protein -
JJB80_RS23260 (JJB80_23255) 15933..16377 + 445 Protein_21 tyrosine-type recombinase/integrase -
JJB80_RS23265 (JJB80_23260) 16636..17625 - 990 WP_210138968.1 RepB family plasmid replication initiator protein -
JJB80_RS23270 (JJB80_23265) 18119..18415 - 297 WP_001687482.1 hypothetical protein -
JJB80_RS23920 18427..18855 + 429 Protein_24 hypothetical protein -
JJB80_RS23275 (JJB80_23270) 18899..19420 - 522 WP_010999942.1 hypothetical protein -
JJB80_RS23280 (JJB80_23275) 20096..20314 + 219 WP_000813641.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JJB80_RS23285 (JJB80_23280) 20316..20621 + 306 WP_001159863.1 type II toxin-antitoxin system toxin CcdB Toxin
JJB80_RS23290 (JJB80_23285) 20623..20913 + 291 WP_001266176.1 hypothetical protein -
JJB80_RS23295 (JJB80_23290) 20910..21431 + 522 WP_000198608.1 hypothetical protein -
JJB80_RS23300 (JJB80_23295) 21466..22248 + 783 WP_000082169.1 site-specific integrase -
JJB80_RS23305 (JJB80_23300) 22257..22808 + 552 WP_000545754.1 EAL domain-containing protein -
JJB80_RS23310 (JJB80_23305) 22995..23483 + 489 WP_001075507.1 CaiF/GrlA family transcriptional regulator -
JJB80_RS23315 (JJB80_23310) 23477..23962 + 486 WP_000905606.1 membrane protein -
JJB80_RS23320 (JJB80_23315) 24239..24526 - 288 WP_071530243.1 hypothetical protein -
JJB80_RS23325 (JJB80_23320) 24682..25242 + 561 WP_000900095.1 inverse autotransporter beta domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - rck / pefD / pefC / pefA / pefB / fdeC / spvC / spvB 1..93939 93939
- flank IS/Tn - - 25309..25659 350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11587.48 Da        Isoelectric Point: 6.9787

>T185735 WP_001159863.1 NZ_CP067092:20316-20621 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Download         Length: 306 bp

>T185735 NZ_CP089012:c4744112-4743195 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8314.16 Da        Isoelectric Point: 4.5203

>AT185735 WP_000813641.1 NZ_CP067092:20096-20314 [Salmonella enterica subsp. enterica serovar Typhimurium]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Download         Length: 219 bp

>AT185735 NZ_CP089012:c4744876-4744118 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTTGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I3W3D5

Antitoxin

Source ID Structure
AlphaFold DB A0A656ICA6

References