Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4724572..4725148 Replicon chromosome
Accession NZ_CP067091
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain ER3625

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag JJB80_RS22790 Protein ID WP_001131963.1
Coordinates 4724572..4724859 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag JJB80_RS22795 Protein ID WP_000063142.1
Coordinates 4724846..4725148 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB80_RS22765 (4722514) 4722514..4722657 + 144 Protein_4452 restriction endonuclease -
JJB80_RS22770 (4722731) 4722731..4723051 + 321 Protein_4453 IS4 family transposase -
JJB80_RS22775 (4723080) 4723080..4723874 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
JJB80_RS22780 (4723895) 4723895..4724140 + 246 WP_116212426.1 hypothetical protein -
JJB80_RS22785 (4724154) 4724154..4724303 + 150 Protein_4456 restriction endonuclease -
JJB80_RS22790 (4724572) 4724572..4724859 + 288 WP_001131963.1 BrnT family toxin Toxin
JJB80_RS22795 (4724846) 4724846..4725148 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
JJB80_RS22800 (4725223) 4725223..4726179 - 957 WP_000187839.1 GTPase -
JJB80_RS22805 (4726190) 4726190..4726393 - 204 WP_000460616.1 YbdD/YjiX family protein -
JJB80_RS22810 (4726488) 4726488..4728638 - 2151 WP_000379927.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T185734 WP_001131963.1 NZ_CP067091:4724572-4724859 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T185734 NZ_CP089012:c4585712-4585428 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT185734 WP_000063142.1 NZ_CP067091:4724846-4725148 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT185734 NZ_CP089012:c4585944-4585702 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References