Detailed information of TA system TA185708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 331127..331752
• Replicon: chromosome
• Accession: NZ_CP067089
• Organism: Breznakiella homolactica strain RmG30

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFL75_RS01680
• Protein ID: WP_215624967.1
• Coordinates: 331357..331752 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JFL75_RS01675
• Protein ID: WP_215624966.1
• Coordinates: 331127..331357 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFL75_RS01660 (JFL75_11945)	327314..327865	+	552	WP_215624963.1	hypothetical protein	-
JFL75_RS01665 (JFL75_11950)	328382..329701	+	1320	WP_215624964.1	hypothetical protein	-
JFL75_RS01670 (JFL75_11955)	329967..331019	+	1053	WP_215624965.1	hypothetical protein	-
JFL75_RS01675 (JFL75_11960)	331127..331357	+	231	WP_215624966.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JFL75_RS01680 (JFL75_11965)	331357..331752	+	396	WP_215624967.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFL75_RS01685 (JFL75_11970)	332163..332525	-	363	WP_215624968.1	helix-turn-helix domain-containing protein	-
JFL75_RS01690 (JFL75_11975)	332691..333200	+	510	WP_215624969.1	NAD(P)H-dependent oxidoreductase	-
JFL75_RS01695 (JFL75_11980)	333912..334433	+	522	WP_215624970.1	hypothetical protein	-
JFL75_RS01700 (JFL75_11985)	334441..334995	+	555	WP_215624971.1	hypothetical protein	-
JFL75_RS01705 (JFL75_11990)	334992..335588	+	597	WP_215624972.1	hypothetical protein	-
JFL75_RS01710 (JFL75_11995)	335711..336157	+	447	WP_215624973.1	MarR family transcriptional regulator	-
JFL75_RS01715 (JFL75_12000)	336154..336732	+	579	WP_215624974.1	flavin reductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	327314..335588	8274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14921.61 Da
• Isoelectric Point: 9.3362

>T185708 WP_215624967.1 NZ_CP067089:331357-331752 [Breznakiella homolactica]
MYLLDTNICIYIIKEQPEAVLKNLRKKRSKGMAISSITLAELEYGVENSKYPEKNKIALIKFLSIISILPFDAKAAEAYG
KIKADLKRRGCIIRPLDILIAAHAKSEDLILVTNNSKEFERVEDLKIENWV

Nucleotide

• Length: 396 bp

>T185708 NZ_CP089001:332559-332927 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8790.73 Da
• Isoelectric Point: 4.3063

>AT185708 WP_215624966.1 NZ_CP067089:331127-331357 [Breznakiella homolactica]
MQTAKLFTNGRSQAVRLPKDYQFNGEDVYIQKVGDAVILFPKDKDWEIFLDGLNSFSDDFLSEGRDQRTEGERETL

Nucleotide

• Length: 231 bp

>AT185708 NZ_CP089001:332341-332562 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA