Detailed information of TA system TA185704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 469451..470049
• Replicon: plasmid pAtAB2-73d
• Accession: NZ_CP067074
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I8E17_RS33260
• Protein ID: WP_209159983.1
• Coordinates: 469756..470049 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I8E17_RS33255
• Protein ID: WP_209159982.1
• Coordinates: 469451..469759 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8E17_RS33240 (I8E17_33050)	465656..468016	-	2361	WP_209159979.1	hypothetical protein	-
I8E17_RS33245 (I8E17_33055)	468166..468744	-	579	WP_209159980.1	PIN domain-containing protein	-
I8E17_RS33250 (I8E17_33060)	468734..469180	-	447	WP_209159981.1	DNA-binding protein	-
I8E17_RS33255 (I8E17_33065)	469451..469759	+	309	WP_209159982.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I8E17_RS33260 (I8E17_33070)	469756..470049	+	294	WP_209159983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I8E17_RS35345	470110..470367	-	258	WP_255610431.1	SIP domain-containing protein	-
I8E17_RS35350	470613..470675	-	63	Protein_457	hypothetical protein	-
I8E17_RS33270 (I8E17_33080)	470702..471355	+	654	WP_229002292.1	ABC transporter permease	-
I8E17_RS33275 (I8E17_33085)	471400..471867	+	468	Protein_459	class I SAM-dependent methyltransferase	-
I8E17_RS33280 (I8E17_33090)	472449..472589	+	141	WP_219511825.1	hypothetical protein	-
I8E17_RS33285 (I8E17_33100)	472756..473760	+	1005	WP_209159984.1	alpha/beta hydrolase	-
I8E17_RS33290 (I8E17_33105)	473744..474157	+	414	WP_209159985.1	DoxX family protein	-
I8E17_RS33295 (I8E17_33110)	474179..474318	+	140	Protein_463	fasciclin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..605540	605540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-52)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10568.08 Da
• Isoelectric Point: 5.7904

>T185704 WP_209159983.1 NZ_CP067074:469756-470049 [Rhizobium sp. AB2/73]
VKLTWSAFALSDRDAIFTYIEADNPAAAVLVDERIVAASRRLLDFPASGRVGRIAGTRELVINGTPYVAVYAVTDTTVRI
LRVLHGAQEWPESVPIS

Nucleotide

• Length: 294 bp

>T185704 NZ_CP088999:19053-19469 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11055.46 Da
• Isoelectric Point: 4.8892

>AT185704 WP_209159982.1 NZ_CP067074:469451-469759 [Rhizobium sp. AB2/73]
MAANALVQTRIDAEVRDRASAVLESMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDVWFRNKVLEALNDTRPDVSDD
EVEAHFAERRAAARLKAGALKS

Nucleotide

• Length: 309 bp

>AT185704 NZ_CP088999:18826-19056 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA