Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 545705..546375 Replicon plasmid pAtAB2-73f
Accession NZ_CP067072
Organism Rhizobium sp. AB2/73

Toxin (Protein)


Gene name vapC Uniprot ID A0A1E4Y5D1
Locus tag I8E17_RS22210 Protein ID WP_069614530.1
Coordinates 545971..546375 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1E4Y5E1
Locus tag I8E17_RS22205 Protein ID WP_069614531.1
Coordinates 545705..545974 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8E17_RS22185 (I8E17_22145) 540746..541459 + 714 WP_069614535.1 FadR/GntR family transcriptional regulator -
I8E17_RS22190 (I8E17_22150) 541431..542858 - 1428 WP_069614534.1 aspartate ammonia-lyase -
I8E17_RS22195 (I8E17_22155) 543133..544584 + 1452 WP_069614533.1 amino acid permease -
I8E17_RS22200 (I8E17_22160) 544581..545540 + 960 WP_069614532.1 asparaginase -
I8E17_RS22205 (I8E17_22165) 545705..545974 + 270 WP_069614531.1 antitoxin Antitoxin
I8E17_RS22210 (I8E17_22170) 545971..546375 + 405 WP_069614530.1 type II toxin-antitoxin system VapC family toxin Toxin
I8E17_RS22215 (I8E17_22175) 546432..546677 - 246 WP_069614529.1 hypothetical protein -
I8E17_RS22220 (I8E17_22180) 546704..547993 - 1290 WP_069614528.1 isocitrate lyase -
I8E17_RS22225 (I8E17_22185) 548199..549611 + 1413 WP_069614527.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA 1..1332287 1332287


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(18-56)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14308.68 Da        Isoelectric Point: 5.1871

>T185700 WP_069614530.1 NZ_CP067072:545971-546375 [Rhizobium sp. AB2/73]
MSKLYMLDTNIVSELARNPQGIVARRIADVGPDAICVSIITAAELRYGCAKKGSPKLLAQIEAILGSIPVLALDVPADAE
YGAIRAELEVAGKPIGPNDLLIAAHAHALEAVLVTANIGEFKRVRDLRVENWLD

Download         Length: 405 bp

>T185700 NZ_CP088997:c4879665-4879381 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.78 Da        Isoelectric Point: 5.6560

>AT185700 WP_069614531.1 NZ_CP067072:545705-545974 [Rhizobium sp. AB2/73]
MRHLRHNEPSPPREAKLFRNNKSQAVRIPADFELPGDRVMIHREGDRLIIEPVRRKSLLDVLASLEPLGPEDQFPDIDDT
LLPAKEIEL

Download         Length: 270 bp

>AT185700 NZ_CP088997:c4879897-4879655 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E4Y5D1

Antitoxin

Source ID Structure
AlphaFold DB A0A1E4Y5E1

References