Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1657459..1658123 Replicon chromosome
Accession NZ_CP067071
Organism Rhizobium sp. AB2/73

Toxin (Protein)


Gene name vapC Uniprot ID A0A1E4Y9V2
Locus tag I8E17_RS08360 Protein ID WP_069613021.1
Coordinates 1657683..1658123 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1E4YAE0
Locus tag I8E17_RS08355 Protein ID WP_069613201.1
Coordinates 1657459..1657686 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8E17_RS08330 (I8E17_08325) 1652869..1653693 - 825 WP_113366607.1 inositol monophosphatase family protein -
I8E17_RS08335 (I8E17_08330) 1653710..1654537 - 828 WP_069613018.1 inositol monophosphatase family protein -
I8E17_RS08340 (I8E17_08335) 1654611..1655498 - 888 WP_069613019.1 tRNA 2-thiocytidine(32) synthetase TtcA -
I8E17_RS08345 (I8E17_08340) 1655559..1656488 - 930 WP_069613200.1 glutaminase -
I8E17_RS08350 (I8E17_08345) 1656560..1657177 - 618 WP_069613020.1 30S ribosomal protein S4 -
I8E17_RS08355 (I8E17_08350) 1657459..1657686 + 228 WP_069613201.1 CopG family transcriptional regulator Antitoxin
I8E17_RS08360 (I8E17_08355) 1657683..1658123 + 441 WP_069613021.1 PIN domain-containing protein Toxin
I8E17_RS08365 (I8E17_08360) 1658131..1659648 - 1518 WP_069613022.1 ATP-binding protein -
I8E17_RS08370 (I8E17_08365) 1659779..1660924 - 1146 WP_069613023.1 alpha-hydroxy acid oxidase -
I8E17_RS08375 (I8E17_08370) 1661010..1661474 - 465 WP_069613024.1 GNAT family N-acetyltransferase -
I8E17_RS08380 (I8E17_08375) 1661477..1662277 - 801 WP_069613025.1 glutamate racemase -
I8E17_RS08385 (I8E17_08380) 1662258..1663100 - 843 WP_069613026.1 RNA methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(2-129)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 15886.09 Da        Isoelectric Point: 6.3690

>T185695 WP_069613021.1 NZ_CP067071:1657683-1658123 [Rhizobium sp. AB2/73]
VTFLLDVNVLIALFDPSHVSHDIAHEWFHSLGGDSWATCPLTENGVVRILSQPSYPNSPGPPSTAALLVAQLRRLPGHQF
WADDISLLDDTLVDLTRILTPGQITDTYLLALAKFHRGQLATLDRRLSTSAVKDGKAALHIISSNA

Download         Length: 441 bp

>T185695 NZ_CP088997:877214-877621 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8214.41 Da        Isoelectric Point: 8.8566

>AT185695 WP_069613201.1 NZ_CP067071:1657459-1657686 [Rhizobium sp. AB2/73]
MRTTLAIDDDVLAAAKAMAEQQHRSIGDVISDLARRSLNRSQPAKERNGIPLLTPKTGNPPVTLDLVNKLRDELP

Download         Length: 228 bp

>AT185695 NZ_CP088997:876967-877233 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E4Y9V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1E4YAE0

References