Detailed information of TA system TA185694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 722962..723521
• Replicon: chromosome
• Accession: NZ_CP067071
• Organism: Rhizobium sp. AB2/73

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I8E17_RS03650
• Protein ID: WP_112724384.1
• Coordinates: 722962..723327 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1E4Y0V5
• Locus tag: I8E17_RS03655
• Protein ID: WP_069616104.1
• Coordinates: 723324..723521 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8E17_RS03630 (I8E17_03625)	718032..719264	-	1233	WP_069616101.1	efflux RND transporter periplasmic adaptor subunit	-
I8E17_RS03635 (I8E17_03630)	719685..720473	-	789	WP_069616102.1	SDR family oxidoreductase	-
I8E17_RS03640 (I8E17_03635)	720762..722426	+	1665	WP_069616103.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
I8E17_RS03645 (I8E17_03640)	722433..722960	+	528	WP_083258374.1	GNAT family N-acetyltransferase	-
I8E17_RS03650 (I8E17_03645)	722962..723327	-	366	WP_112724384.1	type II toxin-antitoxin system VapC family toxin	Toxin
I8E17_RS03655 (I8E17_03650)	723324..723521	-	198	WP_069616104.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I8E17_RS03660 (I8E17_03655)	723624..724526	-	903	WP_069616311.1	DMT family transporter	-
I8E17_RS03665 (I8E17_03660)	724671..725144	+	474	WP_069616105.1	Lrp/AsnC family transcriptional regulator	-
I8E17_RS03670 (I8E17_03665)	725210..726583	-	1374	WP_069616106.1	magnesium transporter	-
I8E17_RS03675 (I8E17_03670)	726938..727951	-	1014	WP_113366344.1	zinc-binding alcohol dehydrogenase family protein	-
I8E17_RS03680 (I8E17_03675)	728057..728461	+	405	WP_069616108.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-47)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13559.68 Da
• Isoelectric Point: 7.4003

>T185694 WP_112724384.1 NZ_CP067071:c723327-722962 [Rhizobium sp. AB2/73]
MILVDTSIWIDHFHTPEARLQSLLEQKQVVVHPFVVGEISLGNLRQYDLVIGSLRRLPAIARASDEDVLYFIRSNKLQGT
GIGYIDAHLAAATILTPGTYLWTRDRRLQRVAENLGIAAPL

Nucleotide

• Length: 366 bp

>T185694 NZ_CP088997:741752-742237 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7045.13 Da
• Isoelectric Point: 6.2935

>AT185694 WP_069616104.1 NZ_CP067071:c723521-723324 [Rhizobium sp. AB2/73]
MRTTVAIDDKLLADAREVTGLAETSAILREALKALVEREAAKTLIRLGGTEPNITAPPRRQFGVE

Nucleotide

• Length: 198 bp

>AT185694 NZ_CP088997:741462-741755 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1E4Y0V5