Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 607459..608069 Replicon chromosome
Accession NZ_CP067071
Organism Rhizobium sp. AB2/73

Toxin (Protein)


Gene name hicA Uniprot ID A0A1E4Y882
Locus tag I8E17_RS03080 Protein ID WP_069613844.1
Coordinates 607815..608069 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1E4Y890
Locus tag I8E17_RS03075 Protein ID WP_069613843.1
Coordinates 607459..607818 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8E17_RS03065 (I8E17_03060) 602696..606169 + 3474 WP_069613841.1 indolepyruvate ferredoxin oxidoreductase family protein -
I8E17_RS03070 (I8E17_03065) 606256..607419 + 1164 WP_069613842.1 isovaleryl-CoA dehydrogenase -
I8E17_RS03075 (I8E17_03070) 607459..607818 - 360 WP_069613843.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I8E17_RS03080 (I8E17_03075) 607815..608069 - 255 WP_069613844.1 type II toxin-antitoxin system HicA family toxin Toxin
I8E17_RS03085 (I8E17_03080) 608130..609737 + 1608 WP_069613845.1 carboxyl transferase domain-containing protein -
I8E17_RS03090 (I8E17_03085) 609748..610131 + 384 WP_069613846.1 RidA family protein -
I8E17_RS03095 (I8E17_03090) 610141..612129 + 1989 WP_069613847.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
I8E17_RS03100 (I8E17_03095) 612126..612995 + 870 WP_069613848.1 hydroxymethylglutaryl-CoA lyase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-81)

Antitoxin

Uncharacterised protein family HicB

(52-102)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9541.08 Da        Isoelectric Point: 10.5590

>T185693 WP_069613844.1 NZ_CP067071:c608069-607815 [Rhizobium sp. AB2/73]
MNSKQRQTLEAIFSNPVKATIKWQDIEALLVAIGCEMSEGSGSRVRFTLSGRTLFIHRPHPKPDTKQYVVRTVREFLTDL
GIKP

Download         Length: 255 bp

>T185693 NZ_CP088997:354089-354436 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13335.86 Da        Isoelectric Point: 4.7352

>AT185693 WP_069613843.1 NZ_CP067071:c607818-607459 [Rhizobium sp. AB2/73]
MRSYKGYTGSVEFDEDDMVFHGRIVGIRDIVTFEAETAQELVQAFRDSVDDYLAFCAKRSQEPDRPFSGNLSLRMTPRLH
EQVVRAAGNKAQSVNQWITDTLTEAASRDAESASMVVKA

Download         Length: 360 bp

>AT185693 NZ_CP088997:354436-354735 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E4Y882

Antitoxin

Source ID Structure
AlphaFold DB A0A1E4Y890

References