Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 250546..251188 Replicon chromosome
Accession NZ_CP067069
Organism Bacillus sp. HNR-4

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag JCR32_RS01405 Protein ID WP_000635963.1
Coordinates 250838..251188 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag JCR32_RS01400 Protein ID WP_000004570.1
Coordinates 250546..250833 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCR32_RS01375 (JCR32_01375) 245872..246834 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
JCR32_RS01380 (JCR32_01380) 246827..247399 - 573 WP_274430642.1 rhomboid family intramembrane serine protease -
JCR32_RS01385 (JCR32_01385) 247492..247851 + 360 WP_000635040.1 holo-ACP synthase -
JCR32_RS01390 (JCR32_01390) 248008..248958 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
JCR32_RS01395 (JCR32_01395) 249077..250246 + 1170 WP_000390601.1 alanine racemase -
JCR32_RS01400 (JCR32_01400) 250546..250833 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
JCR32_RS01405 (JCR32_01405) 250838..251188 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JCR32_RS01410 (JCR32_01410) 251256..253424 + 2169 WP_274430643.1 Tex family protein -
JCR32_RS01415 (JCR32_01415) 253482..253598 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
JCR32_RS01420 (JCR32_01420) 253794..254252 + 459 WP_109631580.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T185689 WP_000635963.1 NZ_CP067069:250838-251188 [Bacillus sp. HNR-4]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T185689 NZ_CP088996:c60792-60481 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT185689 WP_000004570.1 NZ_CP067069:250546-250833 [Bacillus sp. HNR-4]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT185689 NZ_CP088996:c60484-60065 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References