Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5350553..5351178 Replicon chromosome
Accession NZ_CP067060
Organism Klebsiella pneumoniae strain ZJ27003

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag JE026_RS25680 Protein ID WP_012737091.1
Coordinates 5350553..5350936 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JE026_RS25685 Protein ID WP_004150355.1
Coordinates 5350936..5351178 (-) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE026_RS25665 (5347919) 5347919..5348821 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JE026_RS25670 (5348818) 5348818..5349453 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JE026_RS25675 (5349450) 5349450..5350379 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JE026_RS25680 (5350553) 5350553..5350936 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
JE026_RS25685 (5350936) 5350936..5351178 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JE026_RS25690 (5351383) 5351383..5352300 + 918 WP_012737090.1 alpha/beta hydrolase -
JE026_RS25695 (5352314) 5352314..5353255 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JE026_RS25700 (5353300) 5353300..5353737 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JE026_RS25705 (5353734) 5353734..5354594 - 861 WP_004146232.1 virulence factor BrkB family protein -
JE026_RS25710 (5354588) 5354588..5355187 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-110)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T185669 WP_012737091.1 NZ_CP067060:c5350936-5350553 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T185669 NZ_CP088989:c41706-41290 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT185669 WP_004150355.1 NZ_CP067060:c5351178-5350936 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT185669 NZ_CP088989:c41933-41703 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A330XB05


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References