Detailed information of TA system TA185665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2741730..2743318
• Replicon: chromosome
• Accession: NZ_CP067060
• Organism: Klebsiella pneumoniae strain ZJ27003

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A331XLA5
• Locus tag: JE026_RS13240
• Protein ID: WP_014907613.1
• Coordinates: 2741730..2743052 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: JE026_RS13245
• Protein ID: WP_002904790.1
• Coordinates: 2743052..2743318 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE026_RS13225 (2736786)	2736786..2739287	+	2502	WP_014907611.1	fimbria/pilus outer membrane usher protein	-
JE026_RS13230 (2739275)	2739275..2740279	+	1005	WP_014907612.1	fimbrial protein	-
JE026_RS13235 (2740421)	2740421..2741713	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JE026_RS13240 (2741730)	2741730..2743052	-	1323	WP_014907613.1	type II toxin-antitoxin system HipA family toxin	Toxin
JE026_RS13245 (2743052)	2743052..2743318	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JE026_RS13250 (2743556)	2743556..2743882	-	327	WP_004215084.1	hydroxyisourate hydrolase	-
JE026_RS13255 (2743879)	2743879..2744379	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JE026_RS13260 (2744357)	2744357..2745736	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
JE026_RS13265 (2746082)	2746082..2747236	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
JE026_RS13270 (2747217)	2747217..2748140	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T185665 WP_014907613.1 NZ_CP067060:c2743052-2741730 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T185665 NZ_CP088988:4077534-4077752 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT185665 WP_002904790.1 NZ_CP067060:c2743318-2743052 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT185665 NZ_CP088988:4077133-4077507 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A331XLA5

Antitoxin

• AlphaFold DB: A0A0H3GXD8