Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 509419..511045 Replicon plasmid pPlas2
Accession NZ_CP067059
Organism Rahnella aceris strain ZF458

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag JHW33_RS25735 Protein ID WP_200227778.1
Coordinates 509720..511045 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag JHW33_RS25730 Protein ID WP_037033204.1
Coordinates 509419..509715 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHW33_RS25710 504746..505618 + 873 WP_013577985.1 fumarylacetoacetate hydrolase family protein -
JHW33_RS25715 505625..506986 - 1362 WP_200228059.1 PTS transporter subunit EIIC -
JHW33_RS25720 507311..508510 + 1200 WP_037032704.1 2-dehydro-3-deoxy-6-phosphogalactonate aldolase -
JHW33_RS25725 508521..509231 - 711 WP_037032703.1 GntR family transcriptional regulator -
JHW33_RS25730 509419..509715 + 297 WP_037033204.1 helix-turn-helix transcriptional regulator Antitoxin
JHW33_RS25735 509720..511045 + 1326 WP_200227778.1 type II toxin-antitoxin system HipA family toxin Toxin
JHW33_RS25740 511115..513592 - 2478 WP_200227780.1 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family -
JHW33_RS25745 513990..514877 - 888 WP_037033258.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaRAHN-2 hcp/tssD / vipB/tssC / vipA/tssB / mgtB / clpB / AHA_1833 / AHA_1832 / hcp1 / hcp/tssD 1..553387 553387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50300.25 Da        Isoelectric Point: 6.3177

>T185653 WP_200227778.1 NZ_CP067059:509720-511045 [Rahnella aceris]
MRRAQQRLAIWMNGIPVGYWEKQKGEDRLQYSPEWTEDSQGRPLSLSLPFTPGNQVHRGNVVRDYFDNLLPDSEGIRRRL
AVRYHAESLEPFDLLTELGRDCVGAIQLLNVADQPEGIFTIRQRPLSEPEIAGMLRNTAAVLPGQNNQDEDLRLSIAGAQ
EKTALLWHNEQWCLPEGNTPTTHIFKLPLGLVGNMQADMSTSVENEWLCSLILEQYGVPVAKTYIGQFEDQKALIVERFD
RKLSSDEAWWIRLPQEDMCQAMGVSPLRKYQSDGGPGISDIMEILSRSDQAQQDQYQFFRSQIIFWLMAATDGHAKNFSI
AHLSQNRYHMTPLYDILSAWPVIGRGNNKIAWQKCKLAMAVRGSSNYYLISKIQRRHWIKQGEQSGLGKRQIETMIDEII
ELTPAVIENVARQLSKSFPAELADAIFTGMEQQRVRLKAQT

Download         Length: 1326 bp

>T185653 NZ_CP088985:c4663461-4662544 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11257.98 Da        Isoelectric Point: 9.8802

>AT185653 WP_037033204.1 NZ_CP067059:509419-509715 [Rahnella aceris]
MKTVYPLKTLNQLRPLLVGFRKEKGLTQKDVSEKLGVTQQTYARLEANPATASLDRLFRVFNVLGIEMVFSSQSDPERPS
MEEIKPDYLSTPARKEQW

Download         Length: 297 bp

>AT185653 NZ_CP088985:c4664225-4663467 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References