Detailed information of TA system TA185650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 64824..65560
• Replicon: plasmid pPlas1
• Accession: NZ_CP067058
• Organism: Rahnella aceris strain ZF458

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H8P154
• Locus tag: JHW33_RS22730
• Protein ID: WP_014683045.1
• Coordinates: 64824..65303 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: JHW33_RS22735
• Protein ID: WP_200227549.1
• Coordinates: 65300..65560 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW33_RS22700	60454..61908	-	1455	Protein_61	ISNCY family transposase	-
JHW33_RS22705	62059..62430	-	372	WP_200227544.1	type II toxin-antitoxin system death-on-curing family toxin	-
JHW33_RS22710	62427..62648	-	222	WP_200227547.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JHW33_RS22715	62863..63129	-	267	WP_013578404.1	Txe/YoeB family addiction module toxin	-
JHW33_RS22720	63126..63371	-	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JHW33_RS22725	63683..64732	+	1050	WP_014683046.1	tyrosine-type recombinase/integrase	-
JHW33_RS22730	64824..65303	-	480	WP_014683045.1	GNAT family N-acetyltransferase	Toxin
JHW33_RS22735	65300..65560	-	261	WP_200227549.1	DUF1778 domain-containing protein	Antitoxin
JHW33_RS22740	65685..66023	+	339	Protein_69	MFS transporter	-
JHW33_RS22745	66409..67869	+	1461	WP_200227552.1	sensor domain-containing diguanylate cyclase	-
JHW33_RS22750	68402..68578	-	177	WP_200227554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..188256	188256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (26-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17323.02 Da
• Isoelectric Point: 9.5460

>T185650 WP_014683045.1 NZ_CP067058:c65303-64824 [Rahnella aceris]
MSLQLSAPEPLTTAHILDAFGCGETTLDEWLKRRALTNHLNGASRTFVVTAHDKSVVGYYALAAGAVSHREAAGSVRRNM
PDPIPVIVLGRLAVDLRTQGVKLGSSLLQDAVLRVRSIVENTGVRALLVHALHEPAKQFYEHYGFTSSPINPMTLMLRL

Nucleotide

• Length: 480 bp

>T185650 NZ_CP088985:3790844-3791062 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9741.18 Da
• Isoelectric Point: 4.7483

>AT185650 WP_200227549.1 NZ_CP067058:c65560-65300 [Rahnella aceris]
MRDAAINLRALPEQRDLIDQAASLLGKNRSDFMLEVACERAQAILLDQVFFQLDTEKFHEFTAMLDAPHNANQGLERLMA
VKAPWA

Nucleotide

• Length: 261 bp

>AT185650 NZ_CP088985:3790443-3790817 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: H8P154