Detailed information of TA system TA185644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2369973..2370590
• Replicon: chromosome
• Accession: NZ_CP067057
• Organism: Rahnella aceris strain ZF458

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: JHW33_RS10860
• Protein ID: WP_013576617.1
• Coordinates: 2370387..2370590 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: H8NYI6
• Locus tag: JHW33_RS10855
• Protein ID: WP_013576616.1
• Coordinates: 2369973..2370341 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW33_RS10825	2365963..2366217	+	255	WP_013576610.1	type B 50S ribosomal protein L31	-
JHW33_RS10830	2366238..2366378	+	141	WP_013576611.1	type B 50S ribosomal protein L36	-
JHW33_RS10835	2366503..2367381	-	879	WP_134706538.1	metal ABC transporter substrate-binding protein	-
JHW33_RS10840	2367425..2368282	-	858	WP_200227106.1	metal ABC transporter permease	-
JHW33_RS10845	2368279..2369007	-	729	WP_173362112.1	ABC transporter ATP-binding protein	-
JHW33_RS10850	2369390..2369743	+	354	WP_013576615.1	hypothetical protein	-
JHW33_RS10855	2369973..2370341	+	369	WP_013576616.1	Hha toxicity modulator TomB	Antitoxin
JHW33_RS10860	2370387..2370590	+	204	WP_013576617.1	hemolysin expression modulator Hha	Toxin
JHW33_RS10865	2370666..2371622	-	957	WP_013576618.1	LysR family transcriptional regulator	-
JHW33_RS10870	2371762..2373549	+	1788	WP_037036873.1	adenine deaminase	-
JHW33_RS10875	2373627..2374976	+	1350	WP_200227107.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T185644 WP_013576617.1 NZ_CP067057:2370387-2370590 [Rahnella aceris]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T185644 NZ_CP088985:337183-337551 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14391.15 Da
• Isoelectric Point: 4.7281

>AT185644 WP_013576616.1 NZ_CP067057:2369973-2370341 [Rahnella aceris]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT185644 NZ_CP088985:336965-337186 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: H8NYI7

Antitoxin

• AlphaFold DB: H8NYI6