Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5294505..5295130 Replicon chromosome
Accession NZ_CP067045
Organism Klebsiella pneumoniae strain B6753

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IL988_RS25895 Protein ID WP_002882817.1
Coordinates 5294505..5294888 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IL988_RS25900 Protein ID WP_004150355.1
Coordinates 5294888..5295130 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL988_RS25880 (5291871) 5291871..5292773 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IL988_RS25885 (5292770) 5292770..5293405 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IL988_RS25890 (5293402) 5293402..5294331 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IL988_RS25895 (5294505) 5294505..5294888 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IL988_RS25900 (5294888) 5294888..5295130 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
IL988_RS25905 (5295335) 5295335..5296252 + 918 WP_032418237.1 alpha/beta hydrolase -
IL988_RS25910 (5296266) 5296266..5297207 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IL988_RS25915 (5297252) 5297252..5297689 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
IL988_RS25920 (5297686) 5297686..5298546 - 861 WP_004146232.1 virulence factor BrkB family protein -
IL988_RS25925 (5298540) 5298540..5299139 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T185630 WP_002882817.1 NZ_CP067045:c5294888-5294505 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T185630 NZ_CP088982:327907-328275 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT185630 WP_004150355.1 NZ_CP067045:c5295130-5294888 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT185630 NZ_CP088982:327689-327910 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References