Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4682871..4683681 Replicon chromosome
Accession NZ_CP067045
Organism Klebsiella pneumoniae strain B6753

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag IL988_RS23025 Protein ID WP_004178461.1
Coordinates 4682871..4683404 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag IL988_RS23030 Protein ID WP_002887278.1
Coordinates 4683415..4683681 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL988_RS23020 (4681702) 4681702..4682823 + 1122 WP_049182140.1 cupin domain-containing protein -
IL988_RS23025 (4682871) 4682871..4683404 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
IL988_RS23030 (4683415) 4683415..4683681 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
IL988_RS23035 (4683784) 4683784..4685217 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
IL988_RS23040 (4685207) 4685207..4685890 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
IL988_RS23045 (4686062) 4686062..4687447 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
IL988_RS23050 (4687465) 4687465..4687809 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T185628 WP_004178461.1 NZ_CP067045:c4683404-4682871 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T185628 NZ_CP088980:1389248-1389562 [Rhodanobacter denitrificans]
ATGATCTTTGTTGAGACGCCAATCTTCACTGCCCAGATCAAGGATCTGCTGAGCGATGATGATTACGGGGCGTTCCAGCG
CTATCTAGCCGAGAATCCGGAGGCTGGCGACGTCATCAAGGAAACCGGCGGACTACGCAAGATCCGCGTGGCGGCGAAGG
GTCACGGCAAGAGCGGCGGCGCGCGAGTGATCTACTACTACTTCGTTTCTGCCTCGCGTATCGCCATGGTTTTCGCCTAT
CCGAAGAACGTACAAGAAAATCTGACGCCTGCGCAAAAAGCAGCACTAAAGAAAGTCATAGAGCAGTGGAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT185628 WP_002887278.1 NZ_CP067045:c4683681-4683415 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT185628 NZ_CP088980:1389577-1389855 [Rhodanobacter denitrificans]
ATGTTCGACAACCTGATGGAAAGCGTGACCCAGATGGACGAGATTGTCCGGGGTGAGCGCCAGCCGTCGCGCGAGGTCCA
TATCGACGCGCTGGCCGTCAAGGAGATCCGCCGCGCCACTGGACTGTCACAGGCCAAGTTCGCCGGCATCGTCGACGTGC
AGGTCGGCACTCTTCGCAACTGGGAGCAGGGCAGGCGTGAACCCACTGGCCCAGCCAAGGCGCTACTGCGCGCGATCAGC
AAGGACCCCAAGCACGTATTGGAGGCGTTGGCCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References