Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1731468..1732058 Replicon chromosome
Accession NZ_CP067045
Organism Klebsiella pneumoniae strain B6753

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag IL988_RS08375 Protein ID WP_022615588.1
Coordinates 1731726..1732058 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag IL988_RS08370 Protein ID WP_000288812.1
Coordinates 1731468..1731725 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL988_RS08345 (1727379) 1727379..1727453 + 75 Protein_1636 type II toxin-antitoxin system PemK/MazF family toxin -
IL988_RS08350 (1727930) 1727930..1729552 + 1623 WP_227652610.1 hypothetical protein -
IL988_RS08355 (1729631) 1729631..1730187 + 557 Protein_1638 hypothetical protein -
IL988_RS08360 (1730470) 1730470..1730930 + 461 Protein_1639 hypothetical protein -
IL988_RS08365 (1730927) 1730927..1731133 + 207 WP_020805021.1 helix-turn-helix transcriptional regulator -
IL988_RS08370 (1731468) 1731468..1731725 + 258 WP_000288812.1 antitoxin Antitoxin
IL988_RS08375 (1731726) 1731726..1732058 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IL988_RS08385 (1732380) 1732380..1733816 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
IL988_RS08395 (1734182) 1734182..1735636 - 1455 WP_004148975.1 AMP nucleosidase -
IL988_RS08400 (1735766) 1735766..1736011 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T185616 WP_022615588.1 NZ_CP067045:1731726-1732058 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T185616 NZ_CP088975:c4585889-4585644 [Rhodococcus pyridinivorans]
ATGGCGAGGTCGGCTGCACGCGCCATGGCGAGGTCGTTGCCGGAGAAGGTAGCAACTGCGGTATACGAGTTCGTCACAGG
ACCCCTTCTCGAGAATTCGAAGCGGGTGGGGAAGCCGTTGAATCCGCCTCTTGCGCCGGCGTACAGCGCCCGGCGAGGTG
AATATCGCGTCCTGTATCTGATCGATGATGCGAACCGCACGGTCGAGGTCACCGCGATTTCGCACCGTGCCGATGCGTAT
CGATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT185616 WP_000288812.1 NZ_CP067045:1731468-1731725 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT185616 NZ_CP088975:c4586203-4585919 [Rhodococcus pyridinivorans]
ATGACGACCCTTCCTGTTGCCGACGCGCGGGCGCAATTGTCACGACTGGTCGATGAGGCCGAGCGCACACACGAGCGATT
CGAGATCACCCGCAACGGGCACCGCGCCGCGGTGCTTCTCGGTGCCGACGATTACGACGCGATGCAGGAGACGATCGCGG
TGTTGTCGGATACGGATCTGCTGCGGGCGCACGTCGAGGGCCGAACGGAGGTCGAGTCGGGAGACGTCGTCGATGTACAG
GAATTGACGCAGCTGATGCGTGATGCTCGGTCGTCCGAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References