Detailed information of TA system TA185613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3863750..3864667
• Replicon: chromosome
• Accession: NZ_CP067043
• Organism: Bacillus velezensis strain 19573-3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: JHX84_RS19025
• Protein ID: WP_025851786.1
• Coordinates: 3863750..3864496 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JHX84_RS19030
• Protein ID: WP_003154807.1
• Coordinates: 3864497..3864667 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHX84_RS19005 (JHX84_19000)	3859142..3860092	-	951	WP_015388352.1	ring-cleaving dioxygenase	-
JHX84_RS19010 (JHX84_19005)	3860415..3861731	+	1317	WP_003154801.1	amino acid permease	-
JHX84_RS19015 (JHX84_19010)	3862017..3862634	+	618	WP_003154804.1	DUF47 domain-containing protein	-
JHX84_RS19020 (JHX84_19015)	3862647..3863645	+	999	WP_003154805.1	inorganic phosphate transporter	-
JHX84_RS19025 (JHX84_19020)	3863750..3864496	+	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JHX84_RS19030 (JHX84_19025)	3864497..3864667	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JHX84_RS20010	3864764..3864889	+	126	WP_003154809.1	hypothetical protein	-
JHX84_RS19035 (JHX84_19030)	3864925..3865803	-	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
JHX84_RS19040 (JHX84_19035)	3865817..3866080	-	264	WP_003154813.1	phage holin	-
JHX84_RS19045 (JHX84_19040)	3866094..3866357	-	264	WP_003154815.1	hemolysin XhlA family protein	-
JHX84_RS19050 (JHX84_19045)	3866409..3867170	-	762	WP_033575307.1	hypothetical protein	-
JHX84_RS19055 (JHX84_19050)	3867227..3867424	-	198	WP_003154819.1	XkdX family protein	-
JHX84_RS19060 (JHX84_19055)	3867430..3867801	-	372	WP_014304856.1	XkdW family protein	-
JHX84_RS19065 (JHX84_19060)	3867814..3869436	-	1623	WP_069473361.1	pyocin knob domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T185613 WP_025851786.1 NZ_CP067043:3863750-3864496 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T185613 NZ_CP088975:472665-472913 [Rhodococcus pyridinivorans]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT185613 WP_003154807.1 NZ_CP067043:3864497-3864667 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT185613 NZ_CP088975:472910-473164 [Rhodococcus pyridinivorans]
GTGAACATCACCTTCACCGACGAAGCGTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAAACAGCTCGAGCGTGT
CAACGAGCTGGTCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGCTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14