Detailed information of TA system TA185611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2545751..2546402
• Replicon: chromosome
• Accession: NZ_CP067042
• Organism: Lacticaseibacillus rhamnosus strain B6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: JGE02_RS12015
• Protein ID: WP_005686631.1
• Coordinates: 2545751..2546134 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: JGE02_RS12020
• Protein ID: WP_005686632.1
• Coordinates: 2546154..2546402 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGE02_RS12010	2544709..2545236	-	528	WP_005711058.1	QueT transporter family protein	-
JGE02_RS12015	2545751..2546134	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JGE02_RS12020	2546154..2546402	-	249	WP_005686632.1	antitoxin	Antitoxin
JGE02_RS12025	2546514..2547653	-	1140	WP_005710798.1	alanine racemase	-
JGE02_RS12030	2547640..2548014	-	375	WP_005686634.1	holo-ACP synthase	-
JGE02_RS12035	2548183..2549691	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
JGE02_RS12040	2549965..2551353	-	1389	WP_005714110.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T185611 WP_005686631.1 NZ_CP067042:c2546134-2545751 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T185611 NZ_CP088965:1784665-1784910 [Brucella anthropi]
ATGAAGATCGTATGGGATGAACCAAAACGCGAAGCCAATCTTGTTAAGCACGGCATGGATTTTGCATCGCTGACTATCGA
GTTTTTTGAAGCATCCACCATCATCCCGGCTAAGCGAGGCAGGCTTCAAGCTATAGGACGCCTCGCAGACGGTACAATCG
TTGTGATTTTCGTTACCCTCGGCACGGAAGCGCTCTCCGTTATCTCAATGCGCTCCGCCCGAAAGGATGAAAGGAGCCTA
ATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT185611 WP_005686632.1 NZ_CP067042:c2546402-2546154 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT185611 NZ_CP088965:1784910-1785212 [Brucella anthropi]
ATGGCCAACAAATTTAATCCGAAACATGCTGCCGAACATGGTTACACCAAGGAAGACTGGGACGCAGTCTCAGACAACCC
TCCTATCACCAAGGAGGAAATGAAAAGAGCAAAGCCATTCAAGGAAGCGCTGCCAGAACTTTACGAAAGCATTCAACGCT
CACGTGGTCGGCCTAGAGTTGCCAGCCCCAAAGAGGCTGTCACGCTGCGCCTTGACCCTGATGTAATTCAAAAGTTCAAG
GCAACTGGGAAAAACTGGCGTGGGAATATGACTGAGGCCCTACGGAAAGCCGTGGGGCTGTAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19