Detailed information of TA system TA185609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3605917..3606505
• Replicon: chromosome
• Accession: NZ_CP067041
• Organism: Bradyrhizobium diazoefficiens strain 65_7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JIR23_RS16410
• Protein ID: WP_200291019.1
• Coordinates: 3606152..3606505 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JIR23_RS16405
• Protein ID: WP_200291016.1
• Coordinates: 3605917..3606171 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIR23_RS16375	3601475..3602779	-	1305	WP_200291006.1	hypothetical protein	-
JIR23_RS16380	3603058..3603309	+	252	WP_200291007.1	hypothetical protein	-
JIR23_RS16385	3603444..3603653	-	210	Protein_3258	AraC family transcriptional regulator	-
JIR23_RS16390	3603878..3604228	-	351	WP_200291008.1	alkylphosphonate utilization protein	-
JIR23_RS16395	3604327..3605166	-	840	WP_200291010.1	PhzF family phenazine biosynthesis protein	-
JIR23_RS16400	3605377..3605649	+	273	WP_200291013.1	hypothetical protein	-
JIR23_RS16405	3605917..3606171	+	255	WP_200291016.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JIR23_RS16410	3606152..3606505	+	354	WP_200291019.1	endoribonuclease MazF	Toxin
JIR23_RS16415	3606578..3606928	+	351	WP_200291022.1	hypothetical protein	-
JIR23_RS16420	3606852..3607556	+	705	WP_200291025.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JIR23_RS16425	3607591..3608487	-	897	WP_200291028.1	LysR family transcriptional regulator	-
JIR23_RS16430	3608628..3609029	+	402	WP_200291031.1	cupin domain-containing protein	-
JIR23_RS16435	3609040..3610035	+	996	WP_200291033.1	aldo/keto reductase	-
JIR23_RS16440	3610159..3610284	+	126	WP_200300226.1	DUF2312 domain-containing protein	-
JIR23_RS16445	3610542..3611105	+	564	WP_200291036.1	type 1 glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

• Antitoxin PemI-like (1-84)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12592.52 Da
• Isoelectric Point: 5.1165

>T185609 WP_200291019.1 NZ_CP067041:3606152-3606505 [Bradyrhizobium diazoefficiens]
MATKPGDYCPEAGDVIWIDLDPTVGHEQSGRRPAMVVSPRSYNTVAGLCVICPITSRVRGYPFEAVLPEGASVRGVVLAD
QPRSISWELRPITPAGRAPDALLLEVRERLAALLGID

Nucleotide

• Length: 354 bp

>T185609 NZ_CP088954:460383-460772 [Acinetobacter calcoaceticus]
TTGATAAAAGAATTGAATTTTGAGCTGAAATATAGTCGTTTCTCGATAATATTTCAGCTTTTTATTGGCTTGAGTTTAGC
AATCTTGCTGTATCAATTGTTATCACCCATTTGGTGGTTGATTGCTTTCATCCTTTTATCTATTAGTTTTCTATTTTTTT
TAAGACAATCACAAATTTCTCAAATTGCATATTTAGACCAAAAATCGTGGTCAATTGCATATCCCTCACAAAAGATAATT
TCTCGTGTGGAAATTTCTAAAATAATTGATTATCAGCTGTTTATGGTTATTTATCTTGAGGGTGCACCTATAAAGGTAGC
CATAATTTGGTTTGATCAACTTCCTTCGGTGCATTGGAAAAAGCTAAAAGTCTTACAGAAACTTCATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9320.58 Da
• Isoelectric Point: 9.9201

>AT185609 WP_200291016.1 NZ_CP067041:3605917-3606171 [Bradyrhizobium diazoefficiens]
MKVAFQKWGNSLALRIPKAFADEIGARDGRAAEMTVSDGKLVIEIARAQRRKRRYALDDLVAGITPANRHDETDWGPAVG
NEAW

Nucleotide

• Length: 255 bp

>AT185609 NZ_CP088954:460119-460376 [Acinetobacter calcoaceticus]
ATGTCTGACGAAATGACCTTGGAAGAACGTAAAGTAATTTATCGAGCACGCCGCGGCTTAAAAGAAATTGACGTCTATTT
TGACCCTTATGTAAAAAACTATTATCTAAAAGCGGATTCGGCAGAGAAGGCCTTATTTGCAGAGTTAGTTGAACAAGAAG
ATCCTGATTTACTAGATTGGTTCATGGAAGTTTCTGAACCTCCACGTACAGAGTTACGTGAATTTATTTATAAATTAAAA
CAGTACGTTCATGGCTAA