Detailed information of TA system TA185605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 352711..353191
• Replicon: chromosome
• Accession: NZ_CP067037
• Organism: Agrobacterium fabrum strain GV3101::pMP90

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A9CK22
• Locus tag: EML485_RS01750
• Protein ID: WP_010971059.1
• Coordinates: 352711..352980 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: EML485_RS01755
• Protein ID: WP_010971060.1
• Coordinates: 352964..353191 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML485_RS01720	347762..348538	-	777	WP_010971054.1	L,D-transpeptidase	-
EML485_RS01725	348777..349406	+	630	WP_010971055.1	DNA-3-methyladenine glycosylase I	-
EML485_RS01730	349403..349846	+	444	WP_010972617.1	hypothetical protein	-
EML485_RS01735	349872..350915	-	1044	WP_035256280.1	YeiH family protein	-
EML485_RS01740	350997..351914	+	918	WP_010971057.1	LysR family transcriptional regulator	-
EML485_RS01745	351940..352704	-	765	WP_010971058.1	hypothetical protein	-
EML485_RS01750	352711..352980	-	270	WP_010971059.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EML485_RS01755	352964..353191	-	228	WP_010971060.1	ribbon-helix-helix protein, CopG family	Antitoxin
EML485_RS01760	353348..354871	+	1524	WP_010971061.1	histidine--tRNA ligase	-
EML485_RS01765	354876..355244	+	369	WP_010971062.1	VOC family protein	-
EML485_RS01770	355261..356388	+	1128	WP_010971063.1	ATP phosphoribosyltransferase regulatory subunit	-
EML485_RS01775	356385..357077	+	693	WP_010971064.1	ATP phosphoribosyltransferase	-
EML485_RS01780	357142..357588	-	447	WP_006313161.1	DoxX family protein	-
EML485_RS01785	357744..358178	-	435	WP_010971066.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10746.46 Da
• Isoelectric Point: 9.9414

>T185605 WP_010971059.1 NZ_CP067037:c352980-352711 [Agrobacterium fabrum]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAALDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T185605 NZ_CP088952:2292612-2292869 [Massilia sp. DM-R-R2A-13]
ATGCGCAGCGTGAAGTTCACGGATGAGGCTTGGAAGACGTACCTCTTCTGGCAGACCCAGGACAGAAAAACGCTCAAACG
TATCAATGACCTGATCGAGGCCGCGCAAAGAGATCCTTTTGCAGGCTTGGGTAAGCCTGAAGCGCTCAAGCACAGCCTTA
GCGGATACTGGTCCCGGCGAATCGATGAAACCAACAGGCTTGTCTACGAAGTGCAGGATGATGAGCTGATCATCATTTCC
TGCCGCTATCACTACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT185605 WP_010971060.1 NZ_CP067037:c353191-352964 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT185605 NZ_CP088952:2292339-2292611 [Massilia sp. DM-R-R2A-13]
ATGAGAATTGTCAATTTTTCTGATGCCCGCAACAGCCTGCGGGCTGTCATCGACCAAGTGGTTGAGGACGCTGACGTCAC
CGTTATCGCGCGACGGGACGCACCTGACGCCGTGGTGATGTCCCTCGATCATTACAACAGCCTGATGGAGACCGTGCACT
TGCTGAGTTCCCCTGCAAACGCTGCCCATCTTGCCAAATCCATTTCGCAGGCACGCGCAGGCCAAGCGAAGCAAAGGAGA
TTAGTCGGCGCTTCCGACGCCATGGACCCGTAA

Structures

Toxin

• AlphaFold DB: A9CK22

Antitoxin

• AlphaFold DB: A9CK21