Detailed information of TA system TA185599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 611420..612045
• Replicon: chromosome
• Accession: NZ_CP067027
• Organism: Agrobacterium fabrum strain GV2260

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q7D0B1
• Locus tag: EML4058_RS03080
• Protein ID: WP_006311244.1
• Coordinates: 611686..612045 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q7D0B2
• Locus tag: EML4058_RS03075
• Protein ID: WP_010971270.1
• Coordinates: 611420..611686 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML4058_RS03045	606594..606929	-	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	-
EML4058_RS03050	606926..607201	-	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EML4058_RS03055	607309..607917	+	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
EML4058_RS03060	608093..610678	+	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
EML4058_RS03065	610675..611097	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
EML4058_RS03070	611150..611350	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
EML4058_RS03075	611420..611686	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EML4058_RS03080	611686..612045	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EML4058_RS03085	612053..612301	-	249	WP_006311245.1	hypothetical protein	-
EML4058_RS03090	612480..613868	+	1389	WP_169539085.1	MFS transporter	-
EML4058_RS03095	613947..614120	-	174	WP_010971273.1	hypothetical protein	-
EML4058_RS03100	614189..615907	-	1719	WP_035256424.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12857.04 Da
• Isoelectric Point: 8.2782

>T185599 WP_006311244.1 NZ_CP067027:611686-612045 [Agrobacterium fabrum]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Nucleotide

• Length: 360 bp

>T185599 NZ_CP088935:1006760-1007014 [Thauera sp. WB-2]
GTGAAACGCAAACACCAGCGCACCCTTGAACTGATCCATGCCCGGCCGGTCTCGGCCAATGTGCGCTGGGGGGACATCGA
GGCTTTGTTCCGGGAGTTGGGTGCCGAGATTTCGGAGCGCGAAGGTTCTCGGGTGGAGGTGTTCCTGTTCGGTGTGGTGC
GTGTCTTTCATCGCCCGCACCCGTCCCCGGATACCGACAAGGGCGCAGTCGCAGCAATTCGGAAGTGGCTTGAAGAAAAC
GGAGTGCAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9423.02 Da
• Isoelectric Point: 5.1106

>AT185599 WP_010971270.1 NZ_CP067027:611420-611686 [Agrobacterium fabrum]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT185599 NZ_CP088935:1007011-1007346 [Thauera sp. WB-2]
ATGAACACCATGAACATCGGGGGCTACGAGGCCGTGATCACCTTCGATCCGGACATCCAGATGTTTCGCGGCGAGTTCGT
CGGTTTGAATGGTGGTGCGGATTTTTATGCGGCAGATGTGGCTGGACTGAGGCGCGAAGGTGAGGTGTCTCTCCGCATGT
TCCTGGATGCGTGCGAACGGCGGGGCATCGAGCCACGAAAGCGTTTCTCGGGCAAGTTCTCGCTGCGCGTCGATCCCGCT
GTGCACGAGGCGGCGGTCGTCGCTGCCGCTGCGCACGGGCAGAGCTTGAATCAGTGGGCAGCCGAGGCGATCAGGCAGGC
CGCCCGGGCGGGGTGA

Structures

Toxin

• AlphaFold DB: Q7D0B1

Antitoxin

• AlphaFold DB: Q7D0B2