Detailed information of TA system TA185598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 606594..607201
• Replicon: chromosome
• Accession: NZ_CP067027
• Organism: Agrobacterium fabrum strain GV2260

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q7D0B7
• Locus tag: EML4058_RS03045
• Protein ID: WP_010971265.1
• Coordinates: 606594..606929 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A9CJP8
• Locus tag: EML4058_RS03050
• Protein ID: WP_010971266.1
• Coordinates: 606926..607201 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML4058_RS03030	602699..604894	-	2196	WP_035256415.1	penicillin-binding protein	-
EML4058_RS03035	605151..605630	+	480	WP_010971263.1	YcgN family cysteine cluster protein	-
EML4058_RS03040	605742..606566	+	825	WP_010971264.1	class D beta-lactamase	-
EML4058_RS03045	606594..606929	-	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EML4058_RS03050	606926..607201	-	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EML4058_RS03055	607309..607917	+	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
EML4058_RS03060	608093..610678	+	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
EML4058_RS03065	610675..611097	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
EML4058_RS03070	611150..611350	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
EML4058_RS03075	611420..611686	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
EML4058_RS03080	611686..612045	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (23-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12991.86 Da
• Isoelectric Point: 10.0059

>T185598 WP_010971265.1 NZ_CP067027:c606929-606594 [Agrobacterium fabrum]
VTNKKDHGKDAALKRATLPRRSDFTKQFIKDWQRLNNSGRYDMVRLKEIMLLLIANGAPLPTQFRDHELTGDWRDHRECH
VGGDFLLIYTVDEKQNLLIFTRAGTHAELFR

Nucleotide

• Length: 336 bp

>T185598 NZ_CP088935:c692650-692252 [Thauera sp. WB-2]
ATGCTCAAGTACCTGCTCGACACCAACATCGTCATCTACACCATCAAGAACAAGCCTGCCCAGGTGCGCGAACGCTTTCG
CCAGCACGACGGCCAGATGGCCATCTCCAGCGTCAGCCTGGGCGAGCTCGTGTTCGGCGCCGAGCGCTCGGCCCAGCCGG
AGGCGAACCTGGCCGTCGTCGAGGGCTTTGTCGCCCGTGTCGAGTCGCTGGATTTCGATGCCGCCGCCGCCCACCACTTC
GGCCAGATTCGCGCCGAGCTCTGCCGCGCCGGCCGCCCCATCGGCCCCTACGACATGATGATCGCCGCCCACGCGCGCTC
GCGCGGCCTCATCCTGGTGACCAACAACACCTCGGAGTTCGAGCGCGTTGCAGGCTTGCGGCTCGAGAACTGGGCCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9918.40 Da
• Isoelectric Point: 6.3350

>AT185598 WP_010971266.1 NZ_CP067027:c607201-606926 [Agrobacterium fabrum]
MTANAYVRARIDQTLKDDATAVLDRLGLTVSDVMRMMLTRIAREKALPIELTQPNAETLAAIEEARAIAAAGRNRFGTSE
ALFEALDAGKR

Nucleotide

• Length: 276 bp

>AT185598 NZ_CP088935:c692892-692653 [Thauera sp. WB-2]
ATGACGAGCTCCTCGCAAACCACCGTCTTTCGCAGCAACAAGACTCAGGCCGTGCGACTGCCCAAGGCGCTCGAGCTGCC
CGAATCGGTGAAGAAGGTCAGGATCACCGCGATCGGCAATACCCGCATCATCACACCCGCCGAAGCGAGCTGGGACGCCT
GGTTCGACGGCCCCGACGTCAGTGCCGACTTCTTGAGCAGCCGCGACCAGCCTGCGGACCAGGTGCGCGAGGCCTGGTGA

Structures

Toxin

• AlphaFold DB: Q7D0B7

Antitoxin

• AlphaFold DB: A9CJP8