Detailed information of TA system TA185596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 17430..18103
• Replicon: plasmid unnamed2
• Accession: NZ_CP067026
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q52562
• Locus tag: JHZ65_RS28850
• Protein ID: WP_005769733.1
• Coordinates: 17430..17849 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q48B52
• Locus tag: JHZ65_RS28855
• Protein ID: WP_003381346.1
• Coordinates: 17846..18103 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHZ65_RS28830	13469..14116	-	648	WP_005760579.1	histidine phosphatase family protein	-
JHZ65_RS28835	14355..15290	-	936	WP_053481463.1	syringolide biosynthetic protein AvrD1	-
JHZ65_RS28840	15565..16197	-	633	WP_044394008.1	recombinase family protein	-
JHZ65_RS28845	16278..17255	+	978	WP_053486377.1	IS5 family transposase	-
JHZ65_RS28850	17430..17849	-	420	WP_005769733.1	type II toxin-antitoxin system VapC family toxin	Toxin
JHZ65_RS28855	17846..18103	-	258	WP_003381346.1	Arc family DNA-binding protein	Antitoxin
JHZ65_RS28860	18471..19103	+	633	WP_003381804.1	ParA family protein	-
JHZ65_RS28865	19139..19393	+	255	WP_003381801.1	hypothetical protein	-
JHZ65_RS28870	19817..20482	-	666	WP_024647998.1	LexA family transcriptional regulator	-
JHZ65_RS28875	20570..20800	+	231	WP_010215521.1	helix-turn-helix domain-containing protein	-
JHZ65_RS28880	21151..21858	+	708	WP_200380884.1	lytic transglycosylase domain-containing protein	-
JHZ65_RS28885	21858..22193	+	336	WP_200380891.1	conjugal transfer protein	-
JHZ65_RS28890	22206..22607	+	402	Protein_25	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29787	29787
	-	flank	IS/Tn	-	-	16278..17255	977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15138.43 Da
• Isoelectric Point: 4.7010

>T185596 WP_005769733.1 NZ_CP067026:c17849-17430 [Pseudomonas syringae pv. maculicola]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T185596 NZ_CP088935:c511320-511015 [Thauera sp. WB-2]
TTGACGACCTGTAAGCTGCGCTTCCACACCCTGGCCCTCGCCGAATGGCAGAAGCTCGATGGCAGCGTGCGCACGCAGTT
CAAGAAGAAACTGGAGGAGCGCCTGATCACGCCCAGGGTACCCGCCGCTGCGCTGTCGGGCATGGCGGACTGCTACAAGA
TCAAGCTCCGCGCTGCAGGGTATCGATTGGTTTACCGGGTCGATGACGGTGAGGTCTGGGTGACGGTCATCTCGATCGGC
CTGCGGGAAAGGCTCCAAGCCTACACCCGGGCGCAGGCCCGCCTGGACGACCCGCCTCGGGACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9401.62 Da
• Isoelectric Point: 9.0330

>AT185596 WP_003381346.1 NZ_CP067026:c18103-17846 [Pseudomonas syringae pv. maculicola]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT185596 NZ_CP088935:c511552-511307 [Thauera sp. WB-2]
ATGCAAACGATCATGGCCGACCTGACGGTCAGCGTCACCGAGCTCAAGCGCAACTTCGCCAGCATCCTCGCCCAGGCCGA
CGATCAGCCCGTGGCCGTACTCAATCACAACCGGCCCGAGGCCTATCTGCTCTCGGCCGCGCACTACCAACGCCTGCTCA
CCCACCTCGAAGACCTCGAAGACGCCGCCCGTGTGCGCGAGCGCAGCGAAGGCCCGTTCGTCGAGGTGAGCCTTGACGAC
CTGTAA

Structures

Toxin

• AlphaFold DB: Q52562

Antitoxin

• AlphaFold DB: Q48B52