Detailed information of TA system TA185595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 25652..26132
• Replicon: plasmid unnamed1
• Accession: NZ_CP067025
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S6W1B6
• Locus tag: JHZ65_RS28685
• Protein ID: WP_017709581.1
• Coordinates: 25652..25924 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S6VUC4
• Locus tag: JHZ65_RS28690
• Protein ID: WP_015062150.1
• Coordinates: 25908..26132 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHZ65_RS28660	21519..21845	+	327	WP_074929282.1	hypothetical protein	-
JHZ65_RS28665	21842..24013	+	2172	WP_095102578.1	DotA/TraY family protein	-
JHZ65_RS28670	24050..24553	+	504	WP_017709584.1	hypothetical protein	-
JHZ65_RS28675	24885..25139	-	255	WP_080106675.1	hypothetical protein	-
JHZ65_RS28680	25180..25479	-	300	WP_024667341.1	mobilization protein	-
JHZ65_RS28685	25652..25924	-	273	WP_017709581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JHZ65_RS28690	25908..26132	-	225	WP_015062150.1	TraY domain-containing protein	Antitoxin
JHZ65_RS28695	26667..26930	+	264	WP_175526992.1	hypothetical protein	-
JHZ65_RS28700	27739..27963	-	225	WP_074929272.1	hypothetical protein	-
JHZ65_RS28705	28374..28706	+	333	WP_003317539.1	IS66 family insertion sequence hypothetical protein	-
JHZ65_RS28710	28703..29038	+	336	WP_004409766.1	IS66 family insertion sequence element accessory protein TnpB	-
JHZ65_RS28715	29210..29550	-	341	Protein_36	hypothetical protein	-
JHZ65_RS28720	29784..30467	+	684	WP_172456589.1	type III effector	-
JHZ65_RS28725	30521..31066	-	546	WP_011107006.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40085	40085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10233.68 Da
• Isoelectric Point: 8.4933

>T185595 WP_017709581.1 NZ_CP067025:c25924-25652 [Pseudomonas syringae pv. maculicola]
MTWRIEFDPAAEKELKELGDDAAKDVLKFLVGRITNQGEPHSIGSALKGSELGELWKYRVGDYRIVSNIQDVTLTILVVR
LSSQQRGYRR

Nucleotide

• Length: 273 bp

>T185595 NZ_CP088935:121931-122209 [Thauera sp. WB-2]
ATGGAGCTGAAGTGGACCAGCAAGGCGCTTGCCGATCTGGTGCGCCTACATGAGTTCCTCGCGCCGGTGAACAGGCAGGC
CGCCGCAAAGACGGTACAGGGGCTGACCAAGGCCCCGACCATCCTGCTGAGCAACCCGCGCATCGGCGAACAGCTCTTCC
AGTTCGCGCCCCGTGAGGTGCGGCGGATCCTGGTCGCGCAGTACGAGGTGCGCTACGAGATCCGCGACTCGGTCATCTAC
GTCCTGCGGCTGTGGCACACCCGAGAGGACCGCGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8548.63 Da
• Isoelectric Point: 4.3135

>AT185595 WP_015062150.1 NZ_CP067025:c26132-25908 [Pseudomonas syringae pv. maculicola]
MLAIRLAPEIEERLEALAARTGRTKTFYAREAILEHIDDLEDLYLAEQRLGEVRENRSETISLQDVIKSYDLAD

Nucleotide

• Length: 225 bp

>AT185595 NZ_CP088935:121662-121931 [Thauera sp. WB-2]
ATGACGCAAGCCACAACCAAAGTGCTCACCGCCCACGTACCGCTCCCGCTCGCCGAGAAGGTCGATCAGATGGCCGCGAG
GCTGGAGCGCTCGCGCGGCTGGATCATGAAGCAAGCGCTCTCCGCCTGGATTGCCCAGGAGGAAGAGCGCGACCGCCTGA
CCCAGGAGGCGCTCGCCGACGTGGACGCCGGTCTCGTCGTCGACCCTCTCGCGGTGCAGGCCTGGGCTGACAGCCTGGGC
ACTGACCAGCCGCTGCCGCTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S6W1B6

Antitoxin

• AlphaFold DB: S6VUC4