Detailed information of TA system TA185593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 6003678..6004292
• Replicon: chromosome
• Accession: NZ_CP067024
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q88AZ2
• Locus tag: JHZ65_RS26945
• Protein ID: WP_005768484.1
• Coordinates: 6003678..6004028 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: F3ILE1
• Locus tag: JHZ65_RS26950
• Protein ID: WP_005768485.1
• Coordinates: 6004041..6004292 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHZ65_RS26910	5998934..5999422	+	489	WP_005771064.1	type VI secretion system tube protein Hcp	-
JHZ65_RS26915	5999469..5999969	+	501	WP_080546557.1	type VI secretion system amidase effector protein Tae4	-
JHZ65_RS26920	5999969..6000400	+	432	WP_122388671.1	type VI secretion system amidase immunity protein Tai4	-
JHZ65_RS26925	6000474..6000839	-	366	Protein_5306	phosphoenolpyruvate carboxykinase (ATP)	-
JHZ65_RS26930	6001115..6001822	+	708	WP_200380616.1	hypothetical protein	-
JHZ65_RS26935	6001919..6002533	+	615	WP_044392626.1	hypothetical protein	-
JHZ65_RS26940	6002923..6003375	+	453	WP_044392609.1	hypothetical protein	-
JHZ65_RS26945	6003678..6004028	-	351	WP_005768484.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JHZ65_RS26950	6004041..6004292	-	252	WP_005768485.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JHZ65_RS26955	6004590..6006134	-	1545	WP_005613653.1	phosphoenolpyruvate carboxykinase	-
JHZ65_RS26960	6006314..6007216	-	903	WP_005768487.1	Hsp33 family molecular chaperone HslO	-
JHZ65_RS26965	6007377..6007784	-	408	WP_003377907.1	RNA-binding protein S4	-
JHZ65_RS26970	6007952..6008734	+	783	WP_005768488.1	EAL domain-containing protein	-
JHZ65_RS26975	6008784..6009227	+	444	WP_172679536.1	ATP-dependent zinc protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13110.05 Da
• Isoelectric Point: 4.3176

>T185593 WP_005768484.1 NZ_CP067024:c6004028-6003678 [Pseudomonas syringae pv. maculicola]
MNMFALRFTDVAQQSLEDQVEHLAVYQGFSPAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNADGYRIFY
EVRETDDINVIVIVLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T185593 NZ_CP088925:c1086314-1086033 [Rhodanobacter denitrificans]
GTGATAAGGAGCTTCGCTGACAAGGAAACCGCGCGACTGTCCGCGACCGGAACAAGCCGGCGGTTTCCGCCGGACATCGT
GCGGTGGGCGCTGAGTCGTCTGAAATATTTGCATCTCGCCACCTGCCCCGAAGATCTGCGCGAGCCGTTTTCCAATCGCC
TGGAAATGTTGAGCGGCAATCGCGCAGGTCAATGGAGTATCCGCATCAACGATCAGTGGCGGCTGTGCTTCCGTTTCGAG
GGCGGCGATGCCTTCGACGTCGAGATCGTCGACTACCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9488.86 Da
• Isoelectric Point: 9.5019

>AT185593 WP_005768485.1 NZ_CP067024:c6004292-6004041 [Pseudomonas syringae pv. maculicola]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSAEELKARLSRRFA
HKE

Nucleotide

• Length: 252 bp

>AT185593 NZ_CP088925:c1086020-1085715 [Rhodanobacter denitrificans]
ATGTCCATCCACGCCACCACGAACGGCCTGTCGCCCATTCATCCGGGGGAACTCCTGGAAGAAATCCTGTCCGACCACGG
CATGAGCCAGGCGGCATTTGCCCGGGCGATCGGCGTTTCGCCGATGCGTATTTCGCATGTCATCCGCGAGGAGCGCCCCG
TCACGGCCGAACTTGCCCTGCGCTTTGGCCGCGCCTTCGGCCAGAGCCCGCAGTTCTGGATGAACATGCAGACCGACTAC
GACCTCAAGATCGCCGAGCGTGAACTGAAGGCGAGTTTGCGGAAAGTTCTGCCATTGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: Q88AZ2

Antitoxin

• AlphaFold DB: A0A0N0WWE1