Detailed information of TA system TA185592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5144745..5145379
• Replicon: chromosome
• Accession: NZ_CP067024
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JHZ65_RS22985
• Protein ID: WP_003380365.1
• Coordinates: 5144975..5145379 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q888H8
• Locus tag: JHZ65_RS22980
• Protein ID: WP_003380366.1
• Coordinates: 5144745..5144975 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHZ65_RS22955	5139822..5140847	+	1026	WP_005770956.1	TRAP transporter substrate-binding protein	-
JHZ65_RS22960	5140911..5141438	+	528	WP_200380541.1	TRAP transporter small permease	-
JHZ65_RS22965	5141439..5142719	+	1281	WP_005736622.1	TRAP transporter large permease	-
JHZ65_RS22970	5142803..5143774	+	972	WP_200380542.1	DMT family transporter	-
JHZ65_RS22975	5143749..5144630	+	882	WP_200380543.1	aldose 1-epimerase	-
JHZ65_RS22980	5144745..5144975	+	231	WP_003380366.1	antitoxin	Antitoxin
JHZ65_RS22985	5144975..5145379	+	405	WP_003380365.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JHZ65_RS22990	5145567..5146142	-	576	WP_200380544.1	hypothetical protein	-
JHZ65_RS22995	5146567..5147079	+	513	WP_005615033.1	sigma-70 family RNA polymerase sigma factor	-
JHZ65_RS23000	5147079..5147741	+	663	WP_044393634.1	ChrR family anti-sigma-E factor	-
JHZ65_RS23005	5147764..5149020	-	1257	WP_200380545.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
JHZ65_RS23010	5149116..5149598	-	483	WP_002556144.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-34)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14814.09 Da
• Isoelectric Point: 6.8615

>T185592 WP_003380365.1 NZ_CP067024:5144975-5145379 [Pseudomonas syringae pv. maculicola]
MLKYMLDTNICIFTIKNKPVSVREAFNLHHGQLCISAITLMELVYGAEKSSSPERNLAVVEGFAARLELLPYDSAAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Nucleotide

• Length: 405 bp

>T185592 NZ_CP088925:c562223-562047 [Rhodanobacter denitrificans]
ATGAAGTACAGCGAGTTCCAGCGGTGGCTCAGGCAGCAGGGAGTGAGCTTCGAGCCCGGCAAGGGCAGCCACCAGCTGGC
GCGCTACAGAGGCCGGACTTCCGTCTTTCCCTTCCACGGATCGAAAGAGATGCCCGAGCCGCTGCGCAAGAAGATCCTGA
AGGACCTGGGTCTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8593.67 Da
• Isoelectric Point: 4.5557

>AT185592 WP_003380366.1 NZ_CP067024:5144745-5144975 [Pseudomonas syringae pv. maculicola]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPVEQLRESL

Nucleotide

• Length: 231 bp

>AT185592 NZ_CP088925:c562014-561595 [Rhodanobacter denitrificans]
ATGAAATTCCCCGTCAAGCTGACCCGTGCCGATAGCGTGGTGATGGTGACCTGTCCGGACCTGCCGGAGTTTTCCAGCGT
CGGCGACACCGAGGGCGAGGCTCTGCGCGAAGCCGTCGACGGCATCGAAACCACACTGCAGGCGTACATGCAGGACCGGC
GTGACATTCCGCCGCCGGGCGCGCGCAAGCGCGGCCAGCGGCTGGTGGAGCTGCCGCCGCTGGCGGTGGCCAAGCTCGGT
CTGTACCGGGCCATGCGCGAACAGGGGTTGCGCAAGGCTGACCTGGCCCGCCGGCTCGACGTGCATCTGCCCCAGGTCGA
CCGCCTGCTCGACCTGCGCCACAGCTCGAAGCTGGAACAGGTGGAGGCGGCGCTCAACGCGTTGGGCTACCGGATCGAAC
TGGCGGTGCTGGCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0K8LU44