Detailed information of TA system TA185589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 403635..404674
• Replicon: chromosome
• Accession: NZ_CP067024
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JHZ65_RS01845
• Protein ID: WP_122389623.1
• Coordinates: 404099..404674 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: F3IDN7
• Locus tag: JHZ65_RS01840
• Protein ID: WP_005763820.1
• Coordinates: 403635..404102 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHZ65_RS01820	399375..400505	-	1131	WP_122389625.1	Fic family protein	-
JHZ65_RS01825	400752..402050	+	1299	WP_122389624.1	M20 family metallopeptidase	-
JHZ65_RS01830	402221..402814	-	594	WP_103409268.1	hypothetical protein	-
JHZ65_RS01835	402860..403360	-	501	WP_010208159.1	hypothetical protein	-
JHZ65_RS01840	403635..404102	+	468	WP_005763820.1	helix-turn-helix domain-containing protein	Antitoxin
JHZ65_RS01845	404099..404674	+	576	WP_122389623.1	PIN domain-containing protein	Toxin
JHZ65_RS01850	404855..405106	+	252	WP_005763823.1	helix-turn-helix domain-containing protein	-
JHZ65_RS01855	405106..405291	+	186	Protein_369	HipA N-terminal domain-containing protein	-
JHZ65_RS01860	405934..408135	+	2202	WP_024680229.1	DEAD/DEAH box helicase	-
JHZ65_RS01865	408145..408825	+	681	WP_032633385.1	DUF2290 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	402221..419211	16990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21838.01 Da
• Isoelectric Point: 5.0537

>T185589 WP_122389623.1 NZ_CP067024:404099-404674 [Pseudomonas syringae pv. maculicola]
MRHSPFTAFYDANVLYPAPLRDFLMHLALTGVYRARWSSQIHDEWKRNLLINCPELTREQLDRTSALMDKAVPDGLVSDY
QSLIEGLKLPDADDCHVLAAAIKCNASVIVTFNLKDFPKDVLDTFDMEPLHPDDFIADLWDLDKAAVLEAAQRQRASLRN
PAHSARQYLDKLLQQKLPETVKLLSDFEFLI

Nucleotide

• Length: 576 bp

>T185589 NZ_CP088923:c1749157-1748963 [Rhodanobacter thiooxydans]
ATGAACAGCAAGGATCTGATCAGAGAACTGCTCAAGGACGGCTGGATACTCGACCGGATCAACGGTTCCCACCACATCTA
CAAGCACCCGCACAAAGCTGGCCATCTCAGCATCCCTCACCCAGAGAAGGAGCTTGGTACCGGACTGATCCGCCAGATCC
GCAAGGCCGCAGGCCTGTTGAAGGAGACGCCATGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17232.77 Da
• Isoelectric Point: 5.6641

>AT185589 WP_005763820.1 NZ_CP067024:403635-404102 [Pseudomonas syringae pv. maculicola]
MSSANATRTVLPGEKEIAAAVESSRQLAAFLTTKCDTQRIELVDETQQREVVELPMFALRLLGEILSELALGNAVKVVPI
HAELTTQEAADMLNVSRPHLVKMLDQGMLPHTKTGRHRRVKFADLMSYKQQRDQASREAMDELAAQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT185589 NZ_CP088923:c1748966-1748562 [Rhodanobacter thiooxydans]
ATGAAATATCCCATCGCCATCGAAACCGGCAGCGAGACGACCGCCTGGGGTGTTGCCGTGCCGGATCTTCCGGGGTGCTT
CTCTGCCGGCGATTCTCCAGACGAGGCATACACCAAGGCCAGAGAAGCCATTGAGCTATGGCTCAATGCCGCCCTCGATA
CCGGTCAGCCGATTCCCAAGGCAAAAACCTTGGAGGAACACATGGCAGATCCCGAATACAAGGGTTGGGCCTGGGGCGTG
ATTGAAATCGATCTCGCCGATTTCGACGACACCGTGGAGCGCATCAACATCACCGTTCCCAAGCGTGTCCTGAGGCAAAT
CGACAAGTACGTCGCCGTGCGCCACGAAAACCGCTCGGGCTTTCTCGCCAGGGCTGCCCTGGAAGCCATCTACGAAAACG
CGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3M3YRB6