Detailed information of TA system TA185584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 218516..219100
• Replicon: plasmid unnamed2
• Accession: NZ_CP067021
• Organism: Acinetobacter sp. CS-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: JFY49_RS17140
• Protein ID: WP_000286964.1
• Coordinates: 218798..219100 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JFY49_RS17135
• Protein ID: WP_000985609.1
• Coordinates: 218516..218797 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFY49_RS17110	213838..215313	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
JFY49_RS17115	215807..216079	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
JFY49_RS17120	216072..216392	-	321	WP_039253786.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFY49_RS17125	216521..217855	-	1335	WP_108091255.1	ISNCY family transposase	-
JFY49_RS17130	218017..218436	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
JFY49_RS17135	218516..218797	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
JFY49_RS17140	218798..219100	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFY49_RS17145	219249..219779	+	531	WP_000172759.1	hypothetical protein	-
JFY49_RS17150	219868..220182	-	315	WP_004728120.1	BrnA antitoxin family protein	-
JFY49_RS17155	220169..220456	-	288	WP_000438825.1	BrnT family toxin	-
JFY49_RS17160	220697..221467	-	771	WP_032073134.1	hypothetical protein	-
JFY49_RS17165	221685..222850	+	1166	WP_148334530.1	IS3 family transposase	-
JFY49_RS17170	223269..223973	-	705	WP_004897650.1	IS6-like element IS1006 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VI / blaNDM-1 / floR / mph(E) / msr(E) / sul2 / blaOXA-58 / aac(3)-IId	-	1..283930	283930
	-	inside	IScluster/Tn	floR / mph(E) / msr(E) / sul2	-	206845..227467	20622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T185584 WP_000286964.1 NZ_CP067021:c219100-218798 [Acinetobacter sp. CS-2]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T185584 NZ_CP088922:c1130762-1130481 [Rhodanobacter denitrificans]
GTGATAAGGAGCTTCGCTGACAAGGAAACCGCGCGACTGTCCGCGACCGGAACAAGCCGGCGGTTTCCGCCGGACATCGT
GCGGCGGGCGCTGAGTCGTCTGAAATATTTGCATCTCGCCACCTGCCCCGAAGATCTGCGCGAGCCGTTTTCCAATCGCC
TGGAAATGTTGAGCGGCAATCGCGCAGGTCAATGGAGTATCCGCATCAACGATCAGTGGCGGCTGTGCTTCCGTTTCGAG
GGCGGCGATGCCTTCGACGTCGAGATCGTCGACTACCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT185584 WP_000985609.1 NZ_CP067021:c218797-218516 [Acinetobacter sp. CS-2]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT185584 NZ_CP088922:c1130468-1130163 [Rhodanobacter denitrificans]
ATGTCCATCCCCGCCACCACGAACGGCCTGCCGCCCATTCATCCGGGGGAACTCCTGGAAGAAATCCTGTCCGACCACGG
CATGAGCCAGGCGGCATTTGCCCGGGCGATCGGCGTTTCGCCGATGCGTATTTCGCATGTCATCCGCGAGGAGCGCCCCG
TCACGGCCGAACTTGCCCTGCGCTTTGGCCGCGCCTTCGGCCAGAGCCCGCAGTTCTGGATGAACATGCAGACCGACTAC
GACCTCAAGATCGCCGAGCGTGAACTGAAGGCGAGTTTGCGGAAAGTTCTGCCATTGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94