Detailed information of TA system TA185576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 812717..813227
• Replicon: chromosome
• Accession: NZ_CP067014
• Organism: Staphylococcus pasteuri strain FDAARGOS_1069

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: I6I26_RS03910
• Protein ID: WP_176745287.1
• Coordinates: 813078..813227 (+)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A654BGA3
• Locus tag: I6I26_RS03905
• Protein ID: WP_017636753.1
• Coordinates: 812717..813010 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I26_RS03880	807870..808661	+	792	WP_046466905.1	hydroxyethylthiazole kinase	-
I6I26_RS03885	808662..809303	+	642	WP_046466906.1	thiamine phosphate synthase	-
I6I26_RS03890	809392..810261	+	870	WP_017636756.1	membrane protein insertase YidC	-
I6I26_RS03895	810300..810944	-	645	WP_017636755.1	HD domain-containing protein	-
I6I26_RS03900	811044..812528	-	1485	WP_200271339.1	cardiolipin synthase	-
I6I26_RS03905	812717..813010	+	294	WP_017636753.1	copper-sensing transcriptional repressor CsoR	Antitoxin
I6I26_RS03910	813078..813227	+	150	WP_176745287.1	heavy-metal-associated domain-containing protein	Toxin
I6I26_RS03915	813365..813502	+	138	WP_017636751.1	hypothetical protein	-
I6I26_RS03920	813636..814826	-	1191	WP_017636750.1	rod shape-determining protein RodA	-
I6I26_RS03925	815198..816268	+	1071	WP_126567641.1	D-alanine--D-alanine ligase	-
I6I26_RS03930	816284..817645	+	1362	WP_126567642.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (2-47)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5538.24 Da
• Isoelectric Point: 3.9915

>T185576 WP_176745287.1 NZ_CP067014:813078-813227 [Staphylococcus pasteuri]
METILKEMIGVYDANVDLSNGHISVEFETPANLNSIEKEIYDSGFKVLY

Nucleotide

• Length: 150 bp

>T185576 NZ_CP088920:1392158-1392472 [Rhodanobacter denitrificans]
ATGATCTTTGTTGAGACGCCAATCTTCACTGCCCAGATCAAGGATCTGCTGAGCGATGATGATTACGGGGCGTTCCAGCG
CTATCTAGCCGAGAATCCGGAGGCTGGCGACGTCATCAAGGAAACCGGCGGACTACGCAAGATCCGCGTGGCGGCGAAGG
GTCACGGCAAGAGCGGCGGCGCGCGAGTGATCTACTACTACTTCGTTTCTGCCTCGCGTATCGCCATGGTTTTCGCCTAT
CCGAAGAACGTACAAGAAAATCTGACGCCTGCGCAAAAAGCAGCACTAAAGAAAGTCATAGAGCAGTGGAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11231.74 Da
• Isoelectric Point: 6.3270

>AT185576 WP_017636753.1 NZ_CP067014:812717-813010 [Staphylococcus pasteuri]
VTEQDHAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCITEKVNNGHQ
EEAMEELLVTFQKLMKD

Nucleotide

• Length: 294 bp

>AT185576 NZ_CP088920:1392487-1392765 [Rhodanobacter denitrificans]
ATGTTCGACAACCTGATGGAAAGCGTGACCCAGATGGACGAGATTGTCCGGGGTGAGCGCCAGCCGTCGCGCGAGGTCCA
TATCGACGCGCTGGCCGTCAAGGAGATCCGCCGCGCCACTGGACTGTCACAGGCCAAGTTCGCCGGCATCGTCGACGTGC
AGGTCGGCACTCTTCGCAACTGGGAGCAGGGCAGGCGTGAACCCACTGGCCCAGCCAAGGCGCTACTGCGCGCGATCAGC
AAGGACCCCAAGCACGTATTGGAGGCGTTGGCCGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A654BGA3