Detailed information of TA system TA185571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1243353..1244131
• Replicon: chromosome
• Accession: NZ_CP067006
• Organism: Xanthomonas campestris pv. campestris strain 54-2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH269_RS05230
• Protein ID: WP_011038220.1
• Coordinates: 1243353..1243844 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH269_RS05235
• Protein ID: WP_011038219.1
• Coordinates: 1243841..1244131 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH269_RS05215 (JH269_05200)	1239681..1240556	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH269_RS05220 (JH269_05205)	1241206..1241883	+	678	WP_003490678.1	response regulator transcription factor	-
JH269_RS05225 (JH269_05210)	1241876..1243210	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH269_RS05230 (JH269_05215)	1243353..1243844	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH269_RS05235 (JH269_05220)	1243841..1244131	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH269_RS05240 (JH269_05225)	1244206..1244607	-	402	WP_267314850.1	SymE family type I addiction module toxin	-
JH269_RS05245 (JH269_05230)	1245139..1245762	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH269_RS05250 (JH269_05235)	1245776..1246639	-	864	WP_267314851.1	A24 family peptidase	-
JH269_RS05255 (JH269_05240)	1246646..1247905	-	1260	WP_070690003.1	type II secretion system F family protein	-
JH269_RS05260 (JH269_05245)	1248256..1248675	+	420	WP_116670568.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1220895..1285355	64460

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185571 WP_011038220.1 NZ_CP067006:c1243844-1243353 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185571 NZ_CP088919:c2868918-2868604 [Rhodanobacter denitrificans]
ATGATCTTTGTTGAGACGCCAATCTTCACTGCCCAGATCAAGGATCTGCTGAGCGATGATGATTACGGGGCGTTCCAGCG
CTATCTAGCCGAGAATCCGGAGGCTGGCGACGTCATCAAGGAAACCGGCGGACTACGCAAGATCCGCGTGGCGGCGAAGG
GTCACGGCAAGAGCGGCGGCGCGCGAGTGATCTACTACTACTTCGTTTCTGCCTCGCGTATCGCCATGGTTTTCGCCTAT
CCGAAGAACGTACAAGAAAATCTGACGCCTGCGCAAAAAGCAGCACTAAAGAAAGTCATAGAGCAGTGGAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185571 WP_011038219.1 NZ_CP067006:c1244131-1243841 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185571 NZ_CP088919:c2868589-2868311 [Rhodanobacter denitrificans]
ATGTTCGACAACCTGATGGAAAGCGTGACCCAGATGGACGAGATTGTCCGGGGTGAGCGCCAGCCGTCGCGCGAGGTCCA
TATCGACGCGCTGGCCGTCAAGGAGATCCGCCGCGCCACTGGACTGTCACAGGCCAAGTTCGCCGGCATCGTCGACGTGC
AGGTCGGCACTCTTCGCAACTGGGAGCAGGGCAGGCGTGAACCCACTGGCCCAGCCAAGGCGCTACTGCGCGCGATCAGC
AAGGACCCCAAGCACGTATTGGAGGCGTTGGCCGACTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668