Detailed information of TA system TA185569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1422446..1423224
• Replicon: chromosome
• Accession: NZ_CP067005
• Organism: Xanthomonas campestris pv. campestris strain 54-3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH259_RS06025
• Protein ID: WP_011038220.1
• Coordinates: 1422446..1422937 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH259_RS06030
• Protein ID: WP_011038219.1
• Coordinates: 1422934..1423224 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH259_RS06010 (JH259_05990)	1418774..1419649	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH259_RS06015 (JH259_05995)	1420299..1420976	+	678	WP_003490678.1	response regulator transcription factor	-
JH259_RS06020 (JH259_06000)	1420969..1422303	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH259_RS06025 (JH259_06005)	1422446..1422937	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH259_RS06030 (JH259_06010)	1422934..1423224	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH259_RS06035 (JH259_06015)	1423299..1423700	-	402	WP_267314850.1	SymE family type I addiction module toxin	-
JH259_RS06040 (JH259_06020)	1424232..1424855	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH259_RS06045 (JH259_06025)	1424869..1425732	-	864	WP_267314851.1	A24 family peptidase	-
JH259_RS06050 (JH259_06030)	1425739..1426998	-	1260	WP_070690003.1	type II secretion system F family protein	-
JH259_RS06055 (JH259_06035)	1427349..1427768	+	420	WP_116670568.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185569 WP_011038220.1 NZ_CP067005:c1422937-1422446 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185569 NZ_CP088918:c3177743-3177462 [Rhodanobacter denitrificans]
ATGACCATCCAGTCGTTCCGCTGCAAGGATACGCAGGCGCTGTTCGAGCGACGCCGGGTGCGGCGCTTTCTGGTGATCGA
GGCGGCGGCGCGGCGCAAGCTGGAGCTGTTGAATGCGGCGACCCAACTGGACTATCTGCGGGTGCCGCCCGGCAATCGAC
TGGAAGCCTTGGCCGGCGATCGCAAGGGTCAGCACAGCATTCGTGTGAATGACCAGTTCCGGCTGTGCTTCGTGTGGACC
GCCGACGGCCCGGGCCTGGTCGAGATCGTCGATTATCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185569 WP_011038219.1 NZ_CP067005:c1423224-1422934 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185569 NZ_CP088918:c3177452-3177153 [Rhodanobacter denitrificans]
ATGCCCCGCAAACTGCCACCCATCCACCCCGGCGAGATCCTGCGCGAGGAATACATGCTGCCGCTGGAGCTGTCGTCCAA
CGCGCTGGCCCGCGCCCTCGAAGTCACGCCTGCACGTATCAACGAGATCGTGCGCGAGCGGCGCGGCATCAGCGCCGATA
CCGCGCTGCGGCTGGCGCGCTATTTCGGCAATGACGCGCAGAGCTGGCTGAACCTGCAGCAGCGCTACGACATCGAGTGC
GCGCAGGACAGCATCGGCGGCGCACTGAGGAAAATCACGCCCTATCAGGCAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668