Detailed information of TA system TA185567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3764869..3765647
• Replicon: chromosome
• Accession: NZ_CP067004
• Organism: Xanthomonas campestris pv. campestris strain 30-1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH303_RS16500
• Protein ID: WP_011038220.1
• Coordinates: 3765156..3765647 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH303_RS16495
• Protein ID: WP_011038219.1
• Coordinates: 3764869..3765159 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH303_RS16470 (JH303_16480)	3759948..3761150	+	1203	WP_227971345.1	type II secretion system F family protein	-
JH303_RS16475 (JH303_16485)	3761157..3762020	+	864	WP_129588889.1	A24 family peptidase	-
JH303_RS16480 (JH303_16490)	3762034..3762657	+	624	WP_040940796.1	dephospho-CoA kinase	-
JH303_RS16485 (JH303_16495)	3763262..3764370	+	1109	WP_129588887.1	IS3 family transposase	-
JH303_RS16490 (JH303_16500)	3764393..3764794	+	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH303_RS16495 (JH303_16505)	3764869..3765159	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH303_RS16500 (JH303_16510)	3765156..3765647	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH303_RS16505 (JH303_16515)	3765790..3767124	-	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH303_RS16510 (JH303_16520)	3767117..3767794	-	678	WP_003490678.1	response regulator transcription factor	-
JH303_RS16515 (JH303_16525)	3767825..3768298	-	474	WP_012437597.1	hypothetical protein	-
JH303_RS16520 (JH303_16530)	3768521..3769396	-	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185567 WP_011038220.1 NZ_CP067004:3765156-3765647 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185567 NZ_CP088918:276123-276404 [Rhodanobacter denitrificans]
GTGATAAGGAGCTTCGCTGACAAGGAAACCGCGCGACTGTCCGCGACCGGAACAAGCCGGCGGTTTCCGCCGGACATCGT
GCGGTGGGCGCTGAGTCGTCTGAAATATTTGCATCTCGCCACCTGCCCCGAAGATCTGCGCGAGCCGTTTTCCAATCGCC
TGGAAATGTTGAGCGGCAATCGCGCAGGTCAATGGAGTATCCGCATCAACGATCAGTGGCGGCTGTGCTTCCGTTTCGAG
GGCGGCGATGCCTTCGACGTCGAGATCGTCGACTACCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185567 WP_011038219.1 NZ_CP067004:3764869-3765159 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185567 NZ_CP088918:276417-276722 [Rhodanobacter denitrificans]
ATGTCCATCCCCGCCACCACGAACGGCCTGCCGCCCATTCATCCGGGGGAGCTCCTGGAAGAAATCCTGTCCGACCACGG
CATGAGCCAGGCGGCATTTGCCCGGGCGATCGGCGTTTCGCCGATGCGTATTTCGCATGTCATCCGCGAGGAGCGCCCCG
TCACGGCCGAACTTGCCCTGCGCTTTGGCCGCGCCTTCGGCCAGAGCCCGCAGTTCTGGATGAACATGCAGACCGACTAC
GACCTCAAGATCGCCGAGCGTGAACTGAAGGCGAGTTTGCGGAAAGTTCTGCCATTGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668