Detailed information of TA system TA185566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3668955..3669733
• Replicon: chromosome
• Accession: NZ_CP067002
• Organism: Xanthomonas campestris pv. campestris strain 39-3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH263_RS16300
• Protein ID: WP_227971330.1
• Coordinates: 3669242..3669733 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH263_RS16295
• Protein ID: WP_011038219.1
• Coordinates: 3668955..3669245 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH263_RS16270 (JH263_16205)	3664398..3664847	-	450	WP_274514260.1	pilin	-
JH263_RS16275 (JH263_16210)	3665184..3666440	+	1257	WP_075285409.1	type II secretion system F family protein	-
JH263_RS16280 (JH263_16215)	3666447..3667310	+	864	WP_274514261.1	A24 family peptidase	-
JH263_RS16285 (JH263_16220)	3667324..3667947	+	624	WP_274514262.1	dephospho-CoA kinase	-
JH263_RS16290 (JH263_16225)	3668479..3668880	+	402	WP_274514263.1	SymE family type I addiction module toxin	-
JH263_RS16295 (JH263_16230)	3668955..3669245	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH263_RS16300 (JH263_16235)	3669242..3669733	+	492	WP_227971330.1	GNAT family N-acetyltransferase	Toxin
JH263_RS16305 (JH263_16240)	3669876..3671210	-	1335	WP_012437598.1	HAMP domain-containing sensor histidine kinase	-
JH263_RS16310 (JH263_16245)	3671203..3671880	-	678	WP_003490678.1	response regulator transcription factor	-
JH263_RS16315 (JH263_16250)	3671911..3672384	-	474	WP_012437597.1	hypothetical protein	-
JH263_RS16320 (JH263_16255)	3672607..3673482	-	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3655694..3720056	64362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17604.29 Da
• Isoelectric Point: 8.1192

>T185566 WP_227971330.1 NZ_CP067002:3669242-3669733 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPLASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185566 NZ_CP088910:c110179-109877 [Rhodococcus qingshengii]
GTGCGGCCGATTCACGACGCCCAGCTTGGAGGTCTTTGTCCGGTGTTGGTTCTGACGAGGGAATTGGTCCGGCCTCATCT
GGGGACGGTCACTGTTGCTCCCATTCGTTCGGAGGGGCGGGGGTTGTCGACGGAACTGAGTTTAGGCGAAGGCAACGGCG
TCGAGGACGGATCGGTTGTCGACTGCGACGGGGTGACGACGATCCCGGTGTCGGCACTCGGGGTGAATCGAGGTTTTCTC
TTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGTTCTGCGATCAGCATTCGATCTCGAATCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185566 WP_011038219.1 NZ_CP067002:3668955-3669245 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185566 NZ_CP088910:c110395-110180 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGCGAGGTCAGCGGTGGACGCGCGAA
AAGCCGTGCCCAAGTGGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGGCAGCTGGCGGTCCGGGACGTGGAGCTCTTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGAAAGAAGAACTGA

Structures

Antitoxin

• AlphaFold DB: Q8P668