Detailed information of TA system TA185555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1254734..1255512
• Replicon: chromosome
• Accession: NZ_CP066996
• Organism: Xanthomonas campestris pv. campestris strain 33-2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH255_RS05310
• Protein ID: WP_011038220.1
• Coordinates: 1254734..1255225 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH255_RS05315
• Protein ID: WP_011038219.1
• Coordinates: 1255222..1255512 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH255_RS05290 (JH255_05250)	1250985..1251860	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH255_RS05295 (JH255_05255)	1252083..1252556	+	474	WP_012437597.1	hypothetical protein	-
JH255_RS05300 (JH255_05260)	1252587..1253264	+	678	WP_003490678.1	response regulator transcription factor	-
JH255_RS05305 (JH255_05265)	1253257..1254591	+	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH255_RS05310 (JH255_05270)	1254734..1255225	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH255_RS05315 (JH255_05275)	1255222..1255512	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH255_RS05320 (JH255_05280)	1255587..1255988	-	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH255_RS05330 (JH255_05290)	1257724..1258347	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH255_RS05335 (JH255_05295)	1258361..1259224	-	864	WP_129588889.1	A24 family peptidase	-
JH255_RS05340 (JH255_05300)	1259231..1260433	-	1203	WP_227971345.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185555 WP_011038220.1 NZ_CP066996:c1255225-1254734 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185555 NZ_CP088901:c4165779-4165441 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185555 WP_011038219.1 NZ_CP066996:c1255512-1255222 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185555 NZ_CP088901:c4166133-4165804 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668