Detailed information of TA system TA185551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1254520..1255298
• Replicon: chromosome
• Accession: NZ_CP066994
• Organism: Xanthomonas campestris pv. campestris strain 33-5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH276_RS05295
• Protein ID: WP_011038220.1
• Coordinates: 1254520..1255011 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH276_RS05300
• Protein ID: WP_011038219.1
• Coordinates: 1255008..1255298 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH276_RS05275 (JH276_05225)	1250771..1251646	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH276_RS05280 (JH276_05230)	1251869..1252342	+	474	WP_012437597.1	hypothetical protein	-
JH276_RS05285 (JH276_05235)	1252373..1253050	+	678	WP_003490678.1	response regulator transcription factor	-
JH276_RS05290 (JH276_05240)	1253043..1254377	+	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH276_RS05295 (JH276_05245)	1254520..1255011	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH276_RS05300 (JH276_05250)	1255008..1255298	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH276_RS05305 (JH276_05255)	1255373..1255774	-	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH276_RS05315 (JH276_05265)	1257510..1258133	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH276_RS05320 (JH276_05270)	1258147..1259010	-	864	WP_129588889.1	A24 family peptidase	-
JH276_RS05325 (JH276_05275)	1259017..1260219	-	1203	WP_227971345.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185551 WP_011038220.1 NZ_CP066994:c1255011-1254520 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185551 NZ_CP088901:c1017847-1017320 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185551 WP_011038219.1 NZ_CP066994:c1255298-1255008 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185551 NZ_CP088901:c1018134-1017844 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668