Detailed information of TA system TA185548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3776102..3776880
• Replicon: chromosome
• Accession: NZ_CP066993
• Organism: Xanthomonas campestris pv. campestris strain 42-3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH268_RS16590
• Protein ID: WP_011038220.1
• Coordinates: 3776389..3776880 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH268_RS16585
• Protein ID: WP_011038219.1
• Coordinates: 3776102..3776392 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH268_RS16560 (JH268_16520)	3771181..3772383	+	1203	WP_227971345.1	type II secretion system F family protein	-
JH268_RS16565 (JH268_16525)	3772390..3773253	+	864	WP_129588889.1	A24 family peptidase	-
JH268_RS16570 (JH268_16530)	3773267..3773890	+	624	WP_040940796.1	dephospho-CoA kinase	-
JH268_RS16575 (JH268_16535)	3774495..3775603	+	1109	WP_129588887.1	IS3 family transposase	-
JH268_RS16580 (JH268_16540)	3775626..3776027	+	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH268_RS16585 (JH268_16545)	3776102..3776392	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH268_RS16590 (JH268_16550)	3776389..3776880	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH268_RS16595 (JH268_16555)	3777023..3778357	-	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH268_RS16600 (JH268_16560)	3778350..3779027	-	678	WP_003490678.1	response regulator transcription factor	-
JH268_RS16605 (JH268_16565)	3779058..3779531	-	474	WP_012437597.1	hypothetical protein	-
JH268_RS16610 (JH268_16570)	3779754..3780629	-	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185548 WP_011038220.1 NZ_CP066993:3776389-3776880 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185548 NZ_CP088897:c1093-824 [Acinetobacter baumannii]
ATGAAGATTGAGTGGACTGAAACAGCTCGCCAAGATCGTAGAAATATCTATGATTATCTTGAAGAACGCAACCCTATAGC
AGCTATTGAAATTGATGATTTAATTGAAGAAAAGACAGATTTACTTGTTGATAATCGACTGATGGGGCGCACAGGCAGAC
AGAAAGATACTAGGGAGTTAGTGATACATCCGCATTATGTGGTTGTATATGACATCACTGATATAATACGGATACTCAGA
GTGCTACACACATCGCAGGAGTGGTCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185548 WP_011038219.1 NZ_CP066993:3776102-3776392 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185548 NZ_CP088897:c1376-1083 [Acinetobacter baumannii]
ATGACAGAAGCGACTTTTACCTTTCGGGTAGACCATGACCTAAAGCAAGAATTTTCAAGCCTTGCTAAGACTGTTGATCG
GTCGGGGGCGCAGCTAATCCGTGACTTCATGCGTGACTTTGTTAAGAAGCAACAGGAAGCCGCAGACTATGACAAATGGT
TCAAGCAGCAGGTACAGATCGGCTTAAATGAAGCCAATGCAGGCAAGTTGATTCCACATGAGGACGTAAAGGCAGAGTTT
GCCGCTAGACGTGCTGCAACATTGGCGAAACTGGCAGCGAAGAATGAAGATTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668