Detailed information of TA system TA185546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3774626..3775404
• Replicon: chromosome
• Accession: NZ_CP066992
• Organism: Xanthomonas campestris pv. campestris strain 66-2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH285_RS16590
• Protein ID: WP_011038220.1
• Coordinates: 3774913..3775404 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH285_RS16585
• Protein ID: WP_011038219.1
• Coordinates: 3774626..3774916 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH285_RS16560 (JH285_16520)	3769705..3770907	+	1203	WP_227971345.1	type II secretion system F family protein	-
JH285_RS16565 (JH285_16525)	3770914..3771777	+	864	WP_129588889.1	A24 family peptidase	-
JH285_RS16570 (JH285_16530)	3771791..3772414	+	624	WP_040940796.1	dephospho-CoA kinase	-
JH285_RS16575 (JH285_16535)	3773019..3774127	+	1109	WP_129588887.1	IS3 family transposase	-
JH285_RS16580 (JH285_16540)	3774150..3774551	+	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH285_RS16585 (JH285_16545)	3774626..3774916	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH285_RS16590 (JH285_16550)	3774913..3775404	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH285_RS16595 (JH285_16555)	3775547..3776881	-	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH285_RS16600 (JH285_16560)	3776874..3777551	-	678	WP_003490678.1	response regulator transcription factor	-
JH285_RS16605 (JH285_16565)	3777582..3778055	-	474	WP_012437597.1	hypothetical protein	-
JH285_RS16610 (JH285_16570)	3778278..3779153	-	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185546 WP_011038220.1 NZ_CP066992:3774913-3775404 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185546 NZ_CP088896:2377-2664 [Acinetobacter baumannii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATGTCTCTTTCGAAACTGCAAG
CCTTATTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185546 WP_011038219.1 NZ_CP066992:3774626-3774916 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185546 NZ_CP088896:2651-2965 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668