Detailed information of TA system TA185543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3805358..3806136
• Replicon: chromosome
• Accession: NZ_CP066990
• Organism: Xanthomonas campestris pv. campestris strain 42-1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH289_RS16550
• Protein ID: WP_011038220.1
• Coordinates: 3805645..3806136 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH289_RS16545
• Protein ID: WP_011038219.1
• Coordinates: 3805358..3805648 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH289_RS16520 (JH289_16465)	3800822..3801232	-	411	WP_228424134.1	pilin	-
JH289_RS16525 (JH289_16470)	3801584..3802843	+	1260	WP_228424132.1	type II secretion system F family protein	-
JH289_RS16530 (JH289_16475)	3802850..3803713	+	864	WP_228876553.1	A24 family peptidase	-
JH289_RS16535 (JH289_16480)	3803727..3804350	+	624	WP_274398949.1	dephospho-CoA kinase	-
JH289_RS16540 (JH289_16485)	3804882..3805283	+	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH289_RS16545 (JH289_16490)	3805358..3805648	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH289_RS16550 (JH289_16495)	3805645..3806136	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH289_RS16555 (JH289_16500)	3806279..3807613	-	1335	WP_237351399.1	HAMP domain-containing sensor histidine kinase	-
JH289_RS16560 (JH289_16505)	3807606..3808283	-	678	WP_274398952.1	response regulator transcription factor	-
JH289_RS16565 (JH289_16510)	3808933..3809808	-	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185543 WP_011038220.1 NZ_CP066990:3805645-3806136 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185543 NZ_CP088894:3058867-3059046 [Acinetobacter baumannii]
ATGAGCTTCAACGAGTTTAAACGATGGCTAATTGCACAGGGAGTTATCTTTGTGCGTAAAGGTAAGGGATCACATATGAT
TATTGAATTTAACGGTAAGAAGACTGTTTTCCCTAACCACGGGAAAAAAGAAATTCCTGAAGGCACTCGTTTAAAGATCA
AAAAGGATTTGGGGCTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185543 WP_011038219.1 NZ_CP066990:3805358-3805648 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185543 NZ_CP088894:3059132-3059545 [Acinetobacter baumannii]
ATGAAATGTTATGTCAGTATTCATAGGGAGGGAGAGGCTTTTATAGTCAGCTCAAGTGAACTCCCTGAACTAAATAGTGT
AGGCTATACTTTAGAAGAAGCTTTATCAGAAGCTTTGGATGGTATAGAAACAGTATTCGAAATTTATATGGATGAGCGTA
AAGCCATCCCATTACCATCAAAAGGTAAGAAAGGTGAATATGCAGTTCATCTTCCAGTTCGAGTCGCTGCTAAGGTACGT
TTATATAATGAGATGATCTCACAAAACGTTACTAAAGCTGAATTAGCTCGTCGCTTGGGGTGGTTACAGAAGCAAGCCGA
TCGTTTGCTTTCATTAAAGCATTCAACAAAACTAGAATCGATTGAAAGTGCCTTCCAAGCATTAGGCAAGGATCTCGATA
TAGTTATCGCATAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668