Detailed information of TA system TA185539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1177214..1177992
• Replicon: chromosome
• Accession: NZ_CP066987
• Organism: Xanthomonas campestris pv. campestris strain 42-4

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH311_RS05200
• Protein ID: WP_011038220.1
• Coordinates: 1177214..1177705 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH311_RS05205
• Protein ID: WP_011038219.1
• Coordinates: 1177702..1177992 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH311_RS05185 (JH311_05190)	1173542..1174417	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH311_RS05190 (JH311_05195)	1175067..1175744	+	678	WP_274398952.1	response regulator transcription factor	-
JH311_RS05195 (JH311_05200)	1175737..1177071	+	1335	WP_237351399.1	HAMP domain-containing sensor histidine kinase	-
JH311_RS05200 (JH311_05205)	1177214..1177705	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH311_RS05205 (JH311_05210)	1177702..1177992	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH311_RS05210 (JH311_05215)	1178067..1178468	-	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH311_RS05215 (JH311_05220)	1179000..1179623	-	624	WP_274398949.1	dephospho-CoA kinase	-
JH311_RS05220 (JH311_05225)	1179637..1180500	-	864	WP_228876553.1	A24 family peptidase	-
JH311_RS05225 (JH311_05230)	1180507..1181766	-	1260	WP_228424132.1	type II secretion system F family protein	-
JH311_RS05230 (JH311_05235)	1182118..1182528	+	411	WP_228424134.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185539 WP_011038220.1 NZ_CP066987:c1177705-1177214 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185539 NZ_CP088292:c1899673-1899485 [Phyllobacterium sp. T1018]
ATGAAAAGTGGCGATGTTATTGCACTGCTTGAAAAAGACGGGTGGCGCGAGGTCGCTCGAAAGGGAAGCCACGTACAGTT
CAAACATCCTGAAAAGCCCGGCCGGGTGACGGTTCCCCATCCGAAACGCGATATTCCCATTGGCACGCTCCGAAGCTTCG
AGCGGCAATCTGGATTGAGTTTGAGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185539 WP_011038219.1 NZ_CP066987:c1177992-1177702 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185539 NZ_CP088292:c1899481-1899080 [Phyllobacterium sp. T1018]
ATGCAACACTATATCGGATTGATCCATAAGGATGCGGACAGCGACTATGGCGTTTCGTTTCCCGATTTTCCCGGCGTCGT
TACCGCGGGAACGTCCCTGGATGATGCGCACGCCATGGCAGATGAAGCGCTCTCTTTCCATATCGAGGGCCTGATCGAGG
ATGGGGAAGCCATTCCGGAACCGTCGCGCCTCGAAGATATTATGGCCTATCCCGATAATCGCAGCAGCGTCGCCATTCTG
GTCGCCGTCAAGACCGATGCGAAACGCGCCGTACGGGTAAACGTAACCTTGCCCGAAGACATTCTGCAGCAGATCGACAC
CTATGCGGAAGCACATGGATTTACGCGATCGGGCTTCCTCGCACAGGCGGCGAAAAAGGCCATGGAGCGCGATGCAGCGT
GA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668