Detailed information of TA system TA185523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1430027..1430805
• Replicon: chromosome
• Accession: NZ_CP066980
• Organism: Xanthomonas campestris pv. campestris strain 55-1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH267_RS06070
• Protein ID: WP_011038220.1
• Coordinates: 1430027..1430518 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH267_RS06075
• Protein ID: WP_011038219.1
• Coordinates: 1430515..1430805 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH267_RS06055 (JH267_06050)	1426355..1427230	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH267_RS06060 (JH267_06055)	1427880..1428557	+	678	WP_003490678.1	response regulator transcription factor	-
JH267_RS06065 (JH267_06060)	1428550..1429884	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH267_RS06070 (JH267_06065)	1430027..1430518	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH267_RS06075 (JH267_06070)	1430515..1430805	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH267_RS06080 (JH267_06075)	1430880..1431281	-	402	WP_267314850.1	SymE family type I addiction module toxin	-
JH267_RS06085 (JH267_06080)	1431813..1432436	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH267_RS06090 (JH267_06085)	1432450..1433313	-	864	WP_267314851.1	A24 family peptidase	-
JH267_RS06095 (JH267_06090)	1433320..1434579	-	1260	WP_070690003.1	type II secretion system F family protein	-
JH267_RS06100 (JH267_06095)	1434930..1435349	+	420	WP_116670568.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185523 WP_011038220.1 NZ_CP066980:c1430518-1430027 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185523 NZ_CP088281:c199456-199049 [Bradyrhizobium barranii subsp. barranii]
ATGTATCTCGTTGATACCAATGTTGTTTCCGAGGCGCGGCGCGGCTCTCCGCAGGCAACCGGCTGGCTGCGCTCGGTGGA
TCCGGCCAGCGTCTACCTGAGCACCCTCACCCTCGGCGAGATCATGCGCGGCATCGCGCTGAAGCAGAAGTCGGACCCGA
AGGTGGCCGGCCACCTTGCCGAATGGTTGCGCAAGTTGCGCCACGACCACGCCAACCGCATCCTGGCGGTGACCGACCAG
ATCTCCGTCGAGTGGGGTCGCATCGCCGCGATCCGGCCGTGTGGCGATATCGACGGGCTGATCGCCGCAACCGCGATCGT
CCACGACCTCATCCTCGTCACTCGCAATGTGGGGGATTTCGACGATACGGGCGCAACGGTGATCAATCCCTGGGAGGCGT
CGACATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185523 WP_011038219.1 NZ_CP066980:c1430805-1430515 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185523 NZ_CP088281:c199710-199456 [Bradyrhizobium barranii subsp. barranii]
ATGGAATGGCCGCTGCAGGACGCCAAGAACCAGTTTTCCAAGGTGGTGCAGAAGGCCCGCCTGGAAGGCCCGCAGGTCGT
CACCCTGCGCGGGATGCGCGCCGCGGTGGTGCTGTCGGCCGCCGATTACGATGCATTACGGTCCGGCCGGCCGACCCTGG
TCGACGACTTGCTGGCCGGGCCAAGCTGGGATGACGACCTGGCCGAGGCGGTCACGCGGCGCGCCAAGACCCCCAGCCGT
GACGTGGCCTTCTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668