Detailed information of TA system TA185522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3416609..3417387
• Replicon: chromosome
• Accession: NZ_CP066979
• Organism: Xanthomonas campestris pv. campestris strain 55-2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH308_RS15095
• Protein ID: WP_011038220.1
• Coordinates: 3416896..3417387 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH308_RS15090
• Protein ID: WP_011038219.1
• Coordinates: 3416609..3416899 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH308_RS15065 (JH308_15045)	3412065..3412484	-	420	WP_116670568.1	pilin	-
JH308_RS15070 (JH308_15050)	3412835..3414094	+	1260	WP_070690003.1	type II secretion system F family protein	-
JH308_RS15075 (JH308_15055)	3414101..3414964	+	864	WP_267314851.1	A24 family peptidase	-
JH308_RS15080 (JH308_15060)	3414978..3415601	+	624	WP_040940796.1	dephospho-CoA kinase	-
JH308_RS15085 (JH308_15065)	3416133..3416534	+	402	WP_267314850.1	SymE family type I addiction module toxin	-
JH308_RS15090 (JH308_15070)	3416609..3416899	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH308_RS15095 (JH308_15075)	3416896..3417387	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH308_RS15100 (JH308_15080)	3417530..3418864	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH308_RS15105 (JH308_15085)	3418857..3419534	-	678	WP_003490678.1	response regulator transcription factor	-
JH308_RS15110 (JH308_15090)	3420184..3421059	-	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185522 WP_011038220.1 NZ_CP066979:3416896-3417387 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185522 NZ_CP088281:c199456-199049 [Bradyrhizobium barranii subsp. barranii]
ATGTATCTCGTTGATACCAATGTTGTTTCCGAGGCGCGGCGCGGCTCTCCGCAGGCAACCGGCTGGCTGCGCTCGGTGGA
TCCGGCCAGCGTCTACCTGAGCACCCTCACCCTCGGCGAGATCATGCGCGGCATCGCGCTGAAGCAGAAGTCGGACCCGA
AGGTGGCCGGCCACCTTGCCGAATGGTTGCGCAAGTTGCGCCACGACCACGCCAACCGCATCCTGGCGGTGACCGACCAG
ATCTCCGTCGAGTGGGGTCGCATCGCCGCGATCCGGCCGTGTGGCGATATCGACGGGCTGATCGCCGCAACCGCGATCGT
CCACGACCTCATCCTCGTCACTCGCAATGTGGGGGATTTCGACGATACGGGCGCAACGGTGATCAATCCCTGGGAGGCGT
CGACATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185522 WP_011038219.1 NZ_CP066979:3416609-3416899 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185522 NZ_CP088281:c198938-198684 [Bradyrhizobium barranii subsp. barranii]
ATGGCGCTGAGCATCAAGGATCCCGAAACCGAAAAACTCGCGCGCACGCTAGCCGAGCGGACAGGGGAAACCATCACCGT
CGCGACCCGACGGGCGATCGAGGATAGGTTGCGACGGATCGGGGCCGACGCGACCAAGGCCGCGCTGATTGAGGATATGG
AAGCCATTCGGCGCCGCCTCGCTGCCCTCCCGGTCCTCGATCCACGCAGCATGGATGACATCCTCGGGTACGACGAAAAC
GGCTTGCCGGTCTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668