Detailed information of TA system TA185521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): EcnAB/-
• Location: 130997..131363
• Replicon: chromosome
• Accession: NZ_CP066979
• Organism: Xanthomonas campestris pv. campestris strain 55-2

Toxin (Protein)

• Gene name: EcnB
• Uniprot ID: G0CKD4
• Locus tag: JH308_RS00580
• Protein ID: WP_011270059.1
• Coordinates: 131229..131363 (+)
• Length: 45 a.a.

Antitoxin (Protein)

• Gene name: EcnA
• Uniprot ID: -
• Locus tag: JH308_RS00575
• Protein ID: WP_267314524.1
• Coordinates: 130997..131149 (+)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH308_RS00545 (JH308_00545)	126169..126852	-	684	WP_011039005.1	dTMP kinase	-
JH308_RS00550 (JH308_00550)	127187..127729	-	543	WP_012439507.1	GTPase	-
JH308_RS00555 (JH308_00555)	127726..128643	-	918	WP_267314525.1	hypothetical protein	-
JH308_RS00560 (JH308_00560)	128746..129105	-	360	WP_011039002.1	hypothetical protein	-
JH308_RS00565 (JH308_00565)	129188..129649	-	462	WP_228422658.1	roadblock/LC7 domain-containing protein	-
JH308_RS00570 (JH308_00570)	129963..130886	+	924	WP_040942405.1	arginase	-
JH308_RS00575 (JH308_00575)	130997..131149	+	153	WP_267314524.1	entericidin A/B family lipoprotein	Antitoxin
JH308_RS00580 (JH308_00580)	131229..131363	+	135	WP_011270059.1	entericidin A/B family lipoprotein	Toxin
JH308_RS00585 (JH308_00585)	131597..131806	+	210	WP_011038998.1	CsbD family protein	-
JH308_RS00590 (JH308_00590)	131966..133255	+	1290	WP_029217012.1	tryptophan--tRNA ligase	-
JH308_RS00595 (JH308_00595)	133339..134283	+	945	WP_267314523.1	MBL fold metallo-hydrolase	-
JH308_RS00600 (JH308_00600)	134448..136100	+	1653	WP_267315112.1	M28 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Entericidin A/B (23-42)

Antitoxin

• Entericidin A/B (23-42)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4431.27 Da
• Isoelectric Point: 9.3487

>T185521 WP_011270059.1 NZ_CP066979:131229-131363 [Xanthomonas campestris pv. campestris]
MKRAIALLVLSMLSVGMLAGCNTVAGAGKDVQGAGEKVEDAARK

Nucleotide

• Length: 135 bp

>T185521 NZ_CP088281:c198684-198298 [Bradyrhizobium barranii subsp. barranii]
ATGGTGATCGACTCATCCGCCATTCTGGCGATTTTTTTGAATGAACCGGAAGCCAGCGAATTCGAGCATCGCATTGCGGC
CGATCCTGTCCGGTTGATTTCGGCGGCGACGGTGTTGGAAACCGGAATAGGGATCGAGTCAAAGTTGGGCGACGGCGGCG
GCGCCGAGCTTGATCTCTGGCTACACAAGGTTGGTGTCGAGATTGTTCCGGTCACGGCCGGGCATGCGGATCAGGCACGG
CGGGCGTGGCGGCGGTATGGGAAAGGCCGGCATCCCGCCAAACTGAACTTTGGCGATTGCTTTTCTTACGCTCTCGCCGC
CCTCACCGGAGAACCTCTGCTGTTCAAGGGCAGCGATTTCTCGCAGACCGACATCAAGGCGGCCTGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5172.16 Da
• Isoelectric Point: 8.7942

>AT185521 WP_267314524.1 NZ_CP066979:130997-131149 [Xanthomonas campestris pv. campestris]
MKRLLTLMVLGLFSAGMMTGCNTMAGAGKDMQGAGNKVEKTADKCSDGKC

Nucleotide

• Length: 153 bp

>AT185521 NZ_CP088281:c198938-198684 [Bradyrhizobium barranii subsp. barranii]
ATGGCGCTGAGCATCAAGGATCCCGAAACCGAAAAACTCGCGCGCACGCTAGCCGAGCGGACAGGGGAAACCATCACCGT
CGCGACCCGACGGGCGATCGAGGATAGGTTGCGACGGATCGGGGCCGACGCGACCAAGGCCGCGCTGATTGAGGATATGG
AAGCCATTCGGCGCCGCCTCGCTGCCCTCCCGGTCCTCGATCCACGCAGCATGGATGACATCCTCGGGTACGACGAAAAC
GGCTTGCCGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A0H2XDQ0