Detailed information of TA system TA185520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3717120..3717898
• Replicon: chromosome
• Accession: NZ_CP066978
• Organism: Xanthomonas campestris pv. incanae strain 18048

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH290_RS16280
• Protein ID: WP_223646366.1
• Coordinates: 3717407..3717898 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: G0CD67
• Locus tag: JH290_RS16275
• Protein ID: WP_014508712.1
• Coordinates: 3717120..3717410 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH290_RS16250 (JH290_16205)	3712552..3712992	-	441	WP_223646359.1	pilin	-
JH290_RS16255 (JH290_16210)	3713346..3714605	+	1260	WP_223646360.1	type II secretion system F family protein	-
JH290_RS16260 (JH290_16215)	3714612..3715475	+	864	WP_223646361.1	A24 family peptidase	-
JH290_RS16265 (JH290_16220)	3715489..3716112	+	624	WP_223646362.1	dephospho-CoA kinase	-
JH290_RS16270 (JH290_16225)	3716643..3717044	+	402	WP_223646363.1	SymE family type I addiction module toxin	-
JH290_RS16275 (JH290_16230)	3717120..3717410	+	291	WP_014508712.1	DUF1778 domain-containing protein	Antitoxin
JH290_RS16280 (JH290_16235)	3717407..3717898	+	492	WP_223646366.1	GNAT family N-acetyltransferase	Toxin
JH290_RS16285 (JH290_16240)	3718041..3719375	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH290_RS16290 (JH290_16245)	3719368..3720045	-	678	WP_003490678.1	response regulator transcription factor	-
JH290_RS16295 (JH290_16250)	3720695..3721570	-	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3702652..3754995	52343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17610.25 Da
• Isoelectric Point: 8.1192

>T185520 WP_223646366.1 NZ_CP066978:3717407-3717898 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLAVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185520 NZ_CP088280:c8398335-8397415 [Bradyrhizobium barranii subsp. barranii]
ATGGCGCGTGAGCCTCGCAACATCGGCGCATCAGTGCGAGCGCGGCTCCTCGATCGGGCACGTGCCGAGCGATCGGACTT
CCAGATTCTCCTGACGCGATATGCGCTCGAGCGTCTGCTCTACCGGCTAAGCGCGTCGCCGCACCGCGACCGCTTCATCC
TCAAAGGTGCGATGCTCTTCGTGACCTGGATAGCCGATCCGTTTCGGCCTACGCGCGATCTGGATCTGCTTGGCCACGGC
GACCGCGACACCGAAGCCATTGCCGAAACCTTCCGGGCCATTTGTGCGCAGCCGGTCGTCGATGACGGTGTGACGTTTGA
TGTCGCAGCGCTGATGGCGACCCCGATTCGTGAGGAGCTGGAATATGGTGGCGTGCGGGTCCGCACAACGGCAACTATCG
CCGGGGCGCGTATTCCGATCCAGGTCGACATCGGTTTCGGCGACGCCATTACACCCGCGGCGGTCGAGATCGACTATCCC
ACCCTGCTGGACGCCCCAACGCCGCACCTTCGCGCATATCCGGTCGAAACAGTGGTAGCTGAGAAATTCGAAGCGCTCGT
GACGCTCGGCGTGGCCAACAGTCGGCTCAAGGATTTCTATGATCTCTGGGTAATCTCCCGAACATTCGAGCTTCGCCGGG
CCGCTCTTGCCGAGGCAATTCAGCGGACCTTCGAGCGACGCGGAACGGTCCTCCCATCCGTTGTCCCGGTCGGTCTGACC
GATGAATTCGCGGAAACATGGGCCGCCCAGTGGCGCGCCTTCCTGGAGCGCGATCGGATGGCAGCCGCACCGGATGCTTT
TGCCGTTACCGTCGCCGATCTCCGCCTCTTCTTGATGCCCCTGGTCGTCGGATTGAAAGAAGAACGAATTTGGCCGCCGA
GCGGGCCATGGTCGCCGGGAGCAGCAATCGATGAGGCATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10648.20 Da
• Isoelectric Point: 10.5480

>AT185520 WP_014508712.1 NZ_CP066978:3717120-3717410 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLIAASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185520 NZ_CP088280:c8398945-8398325 [Bradyrhizobium barranii subsp. barranii]
ATGCCGTCCACCAGCGCCCAGCGACTTCGCGCGCTCGACCTCCTGAAGACCCGAGGAATGCTCCGGCTGAAGGATTTCAT
CGCCGAAGGCATCGGCCCGGAGACGCTTGCCCGTCTTGTTCGGGAGGAGGCAGTCGTCCGCCCTGCACGTGGCCTCTATC
AACTCCCCGACGCCCACCTCGAGGCTGCCCATACCCTCGCCGAGGCAGCAGTTCTGGTTCCAAAGGGTGTGGTCTGTCTT
ACCTCGGCCCTCCAATACCACGAACTGACGCTGCAGATGCCGTCGGCTGTATGGATGGCGATCGATCGCTCCGCCTGGCG
ACCCAAGATCGATTATCCACCTATCCGGTTCGTGCGATTCACCGGATCGGCGCTTACCGAAGGGATCGAGCGTCACCGCA
TTGAGAATGTCGACGTTCCTATCACGGATCCGGCGAGGACAATTGTCGATTGCTTTCGCTACCGAACCAAGATCGGCCTT
GACGTCGCGATGGAGGGGCTGCGCGAAGGCCTCCGCCGTCGCCGATGCAAGCCGGATCAACTCTGGCATTACGCTCGCAA
GGCGCGGGTCTGGTCGGTCATGCGTCCCTACGTCGAAGCAATGGTGTCCGATGGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3E1LB51