Detailed information of TA system TA185516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1331832..1332610
• Replicon: chromosome
• Accession: NZ_CP066975
• Organism: Xanthomonas campestris pv. campestris strain 10006

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH265_RS05680
• Protein ID: WP_011038220.1
• Coordinates: 1331832..1332323 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH265_RS05685
• Protein ID: WP_011038219.1
• Coordinates: 1332320..1332610 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH265_RS05660 (JH265_05650)	1328083..1328958	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH265_RS05665 (JH265_05655)	1329181..1329654	+	474	WP_274503106.1	hypothetical protein	-
JH265_RS05670 (JH265_05660)	1329685..1330362	+	678	WP_003490678.1	response regulator transcription factor	-
JH265_RS05675 (JH265_05665)	1330355..1331689	+	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH265_RS05680 (JH265_05670)	1331832..1332323	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH265_RS05685 (JH265_05675)	1332320..1332610	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH265_RS05690 (JH265_05680)	1332685..1333086	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH265_RS05695 (JH265_05685)	1333618..1334241	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH265_RS05700 (JH265_05690)	1334255..1335118	-	864	WP_011038216.1	A24 family peptidase	-
JH265_RS05705 (JH265_05695)	1335125..1336381	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH265_RS05710 (JH265_05700)	1336708..1337148	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185516 WP_011038220.1 NZ_CP066975:c1332323-1331832 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185516 NZ_CP088275:87154-87489 [Phyllobacterium sp. T1293]
ATGCCCGCCTACGTGCCGGATGCCGGCGATATTGTTTGGCTGCATTTCAACCCACAAGCAGGTCACGAACAGGCGGGGCA
TCGCCCCGCAGTTGTACTCAGTCCTGCCAGCTACAACGGCAGGATCGGTTTGATGCTTTGCTGCCCCATGACCACACAGA
TCAAGGGGTATCCATTCGAGGTAGCAATTGCGAGTGATCGACCCAGCGCTGTGCTTGCCGATCAGGTCAAGAGTCTAGAT
TGGCGTGTCCGTCGTGCCACTTACAAAGGCAAGATTTCCGCGAGTGAACTCGCCGAAATTCGCAATAAAGCCGCCGCGCT
GATTGGCAAACGCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185516 WP_011038219.1 NZ_CP066975:c1332610-1332320 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185516 NZ_CP088275:86900-87154 [Phyllobacterium sp. T1293]
ATGGAGTATGTTATGCGTGTAACGGTTAAAAAATGGGGCAACAGTGCTTCTGTACGCATACCGGCTGCTGTCATGGCGGC
AGCTCGACTGGAGCTCGACACGTTGGTTGATGTGCGCGAAGAAGAAGGGCTGATTATTATCGAGCCAGTCCGGCAGGTCG
AATATGATCTGGATAGGTTGATCGAAGGCATAACACCTGAAAATATACATTCTGAGGCCGATTTCGGCGATCCTGTCGGC
AAGGAAGCACTTTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668