Detailed information of TA system TA185513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1331868..1332646
• Replicon: chromosome
• Accession: NZ_CP066972
• Organism: Xanthomonas campestris pv. campestris strain 10008

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH312_RS05675
• Protein ID: WP_011038220.1
• Coordinates: 1331868..1332359 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH312_RS05680
• Protein ID: WP_011038219.1
• Coordinates: 1332356..1332646 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH312_RS05655 (JH312_05645)	1328119..1328994	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH312_RS05660 (JH312_05650)	1329217..1329690	+	474	WP_274503106.1	hypothetical protein	-
JH312_RS05665 (JH312_05655)	1329721..1330398	+	678	WP_003490678.1	response regulator transcription factor	-
JH312_RS05670 (JH312_05660)	1330391..1331725	+	1335	WP_162808569.1	HAMP domain-containing sensor histidine kinase	-
JH312_RS05675 (JH312_05665)	1331868..1332359	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH312_RS05680 (JH312_05670)	1332356..1332646	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH312_RS05685 (JH312_05675)	1332721..1333122	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH312_RS05690 (JH312_05680)	1333654..1334277	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH312_RS05695 (JH312_05685)	1334291..1335154	-	864	WP_011038216.1	A24 family peptidase	-
JH312_RS05700 (JH312_05690)	1335161..1336417	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH312_RS05705 (JH312_05695)	1336744..1337184	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185513 WP_011038220.1 NZ_CP066972:c1332359-1331868 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185513 NZ_CP088273:c3246853-3246578 [Phyllobacterium sp. T1293]
ATGTCCAGATTTGAGTGGAATGAAGACAAGGCCAAAAGCAATATACGCAAACACGGTGTAACTTTCGAATTAGCAGACCG
TTTCGAGTTCTGTACGGCTATGGAACAGTTGGACGACCGGGAGAATTACCGGGAAGAAAGGACGGTCGCCATTGGTTTCA
TAGGGAACGTTCTTTTCACCCTCACTTATACTGAGCGTGGTAGCAAAATTAGAGTTATCTCCTTGCGAAAATCATCGACT
AGCGAGATCAAGCGATATGCCAGAGAATATGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185513 WP_011038219.1 NZ_CP066972:c1332646-1332356 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185513 NZ_CP088273:c3246585-3246289 [Phyllobacterium sp. T1293]
ATGACTGAGAAACAACGTGCCGGGCATAGAGCGCGCATTCAGGCGGCTCTGGATAGTATTTCTCCGGAAGAAGACGCAGC
GCTTACCAAAGCGGCTTTGGACGATCCGGACACTGTCCTCATCACCGAGTTGAGCAGACGCAAGCCCGGTCGACCACTTG
CGGATATTACAAAAACCCCGGTCTCCATTCGCCTTAGCCCGGATGTGGTTGATTATTTCCGTTCGAGCGGGCCCGGTTGG
CAGAGCCGTATTGATGATGTCCTGCGCAAAGCAGCAGGGTTGAAAAAACGCGCGTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668