Detailed information of TA system TA185511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1270378..1271156
• Replicon: chromosome
• Accession: NZ_CP066970
• Organism: Xanthomonas campestris pv. campestris strain 11085

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH300_RS05335
• Protein ID: WP_011038220.1
• Coordinates: 1270378..1270869 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH300_RS05340
• Protein ID: WP_011038219.1
• Coordinates: 1270866..1271156 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH300_RS05320 (JH300_05290)	1266706..1267581	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH300_RS05325 (JH300_05295)	1268231..1268908	+	678	WP_003490678.1	response regulator transcription factor	-
JH300_RS05330 (JH300_05300)	1268901..1270235	+	1335	WP_057671653.1	HAMP domain-containing sensor histidine kinase	-
JH300_RS05335 (JH300_05305)	1270378..1270869	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH300_RS05340 (JH300_05310)	1270866..1271156	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH300_RS05345 (JH300_05315)	1271231..1271632	-	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH300_RS05350 (JH300_05320)	1272164..1272787	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH300_RS05355 (JH300_05325)	1272801..1273664	-	864	WP_057671659.1	A24 family peptidase	-
JH300_RS05360 (JH300_05330)	1273671..1274933	-	1263	WP_057671824.1	type II secretion system F family protein	-
JH300_RS05365 (JH300_05335)	1275275..1275703	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1228425..1284324	55899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185511 WP_011038220.1 NZ_CP066970:c1270869-1270378 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185511 NZ_CP088273:c2338543-2338361 [Phyllobacterium sp. T1293]
ATGGAGCGTGACAGCCGCAAGATCATTAAGAGACTGAAGGACGAAGGTTTCGAACTGGTCTCTGTCAGTGGTTCCCATCA
CAAGTTTCGCAAAGGCACCCTTACTTTGATCGTGCCTCATCCAAAGAAGGATCTGCCGATCGGCACGGCCCGGTCAATAG
CGAAAGATGCAGGCTGGCTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185511 WP_011038219.1 NZ_CP066970:c1271156-1270866 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185511 NZ_CP088273:c2338323-2337931 [Phyllobacterium sp. T1293]
ATGCGTCACTATTTTGCATTGGTTCACAAGGATGAAGACAGCGCGTTCGGCATTCAGTTCCCTGATATCAAAGGGGTCTA
TTCGGCAGCAGATAAAGCCGACGATATCGTCCGCAATGCTGTCGAAGCACTCCGCCTATATGCCGAGGATGCGGAGCTTC
CAGCCCCATCGAGCCATGGGGACATTTTAGCACGGCAAGACATCAGGGATGAACTCGCGGCGGGCGCCTATCTGGTTTTC
GTACCACTGATTGACAATGATACGGCGGTTGTCAGAGCCAACGTCACGTTTGAGCGCGGCCTGTTGCGTGCTATTGACGA
TACCGTCAAGGCGCGAGGTACGACACGCTCCGCCTTTCTGGCCAGTGCCGCCAAGCGCGAGATTGAAGCTTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668