Detailed information of TA system TA185510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1376421..1377199
• Replicon: chromosome
• Accession: NZ_CP066969
• Organism: Xanthomonas campestris strain 10-16

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH307_RS06180
• Protein ID: WP_076038486.1
• Coordinates: 1376421..1376912 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH307_RS06185
• Protein ID: WP_011038219.1
• Coordinates: 1376909..1377199 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH307_RS06165 (JH307_06160)	1372749..1373624	+	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-
JH307_RS06170 (JH307_06165)	1374274..1374951	+	678	WP_003490678.1	response regulator transcription factor	-
JH307_RS06175 (JH307_06170)	1374944..1376278	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH307_RS06180 (JH307_06175)	1376421..1376912	-	492	WP_076038486.1	GNAT family N-acetyltransferase	Toxin
JH307_RS06185 (JH307_06180)	1376909..1377199	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH307_RS06190 (JH307_06185)	1377274..1377675	-	402	WP_274509580.1	SymE family type I addiction module toxin	-
JH307_RS06195 (JH307_06190)	1378207..1378830	-	624	WP_076038488.1	dephospho-CoA kinase	-
JH307_RS06200 (JH307_06195)	1378844..1379707	-	864	WP_040940797.1	A24 family peptidase	-
JH307_RS06205 (JH307_06200)	1379714..1380970	-	1257	WP_076037882.1	type II secretion system F family protein	-
JH307_RS06210 (JH307_06205)	1381314..1381721	+	408	WP_076038490.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1301099..1398707	97608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17652.33 Da
• Isoelectric Point: 8.1192

>T185510 WP_076038486.1 NZ_CP066969:c1376912-1376421 [Xanthomonas campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPLVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185510 NZ_CP088273:c2254942-2254691 [Phyllobacterium sp. T1293]
ATGAAGAAGATTGCCTATAGTAAGTCTGCATTGAAGGTGCTCTTGCGCATTCCTGTGAATGTCGCCAATCGCATTCGTTC
GAAAATTGAACAATATGCTGATGATCCGGCGTTGCAGTCTATGAACGTTACCAAACTGCAAGGGCGGAATGGCTATCGGC
TGCGTGTAGGCGACTGGCGGGTGATTTTCGACGACCATGGCAACGTCATGGATATTTTGGACATTGGCCCGCGCGGCGGC
ATTTATGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185510 WP_011038219.1 NZ_CP066969:c1377199-1376909 [Xanthomonas campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185510 NZ_CP088273:c2254698-2254300 [Phyllobacterium sp. T1293]
ATGAATGAGATAGCTATGGGCAAGACAGCACCGTTCACAATCGTCAAAACACCTGCCAACGAAGAAATGGTCGTGATCCC
ACGCACCCTTTACGACAGGCTCGTCAGCGATGCCGAAATGGCGCGGGATCTTGCTGCTTATGACAAGGTCAAGGCACGGA
TTGATGCTGGCGAAGAGGAGCTGGTGCCATCGGAAATTGTTGAGCGCATATTGGGCGGCGAAAACAAGGTTCGCGTCTGG
CGCGAGTATCGTGGCCTTTCAAATCAGGAACTGGCAGAGAAAGTTGGTATCAGCGCTCCTTATTTATCTGATATCCAGAA
CGGCAAAAAAGAGGGCCGTCTCTCAACTCTGAAGAAAATCGCCGCAGTTCTTCGCGTCGATCTCGATGACCTTGTATGA

Structures

Antitoxin

• AlphaFold DB: Q8P668