Detailed information of TA system TA185508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1152855..1153633
• Replicon: chromosome
• Accession: NZ_CP066968
• Organism: Xanthomonas campestris pv. incanae strain 16-8

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH274_RS04915
• Protein ID: WP_274395994.1
• Coordinates: 1152855..1153346 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH274_RS04920
• Protein ID: WP_011038219.1
• Coordinates: 1153343..1153633 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH274_RS04900 (JH274_04890)	1149182..1150057	+	876	WP_076054142.1	30S ribosomal protein S6--L-glutamate ligase	-
JH274_RS04905 (JH274_04895)	1150708..1151385	+	678	WP_003490678.1	response regulator transcription factor	-
JH274_RS04910 (JH274_04900)	1151378..1152712	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH274_RS04915 (JH274_04905)	1152855..1153346	-	492	WP_274395994.1	GNAT family N-acetyltransferase	Toxin
JH274_RS04920 (JH274_04910)	1153343..1153633	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH274_RS04925 (JH274_04915)	1153709..1154110	-	402	WP_274395995.1	SymE family type I addiction module toxin	-
JH274_RS04930 (JH274_04920)	1154642..1155265	-	624	WP_116670565.1	dephospho-CoA kinase	-
JH274_RS04935 (JH274_04925)	1155279..1156142	-	864	WP_040940797.1	A24 family peptidase	-
JH274_RS04940 (JH274_04930)	1156149..1157408	-	1260	WP_274395996.1	type II secretion system F family protein	-
JH274_RS04945 (JH274_04935)	1157763..1158188	+	426	WP_274395997.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1143873..1171630	27757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17606.25 Da
• Isoelectric Point: 8.1192

>T185508 WP_274395994.1 NZ_CP066968:c1153346-1152855 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALVQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185508 NZ_CP088273:c1880746-1880528 [Phyllobacterium sp. T1293]
ATGGGGGAAACCGAGGCACGGCGTATCATGGCATTCTTGGGGACCCGGATTGCCTTGCTGGACGATCCTCGCCAGACAGG
GAAAACGCTGCGAGGTGCAACGTTAGGCAGTCTATGGCGATATCGTGTGGGAGATTATCGCATCATCTGCGAAATACAGG
ATCAAAAACTCGTCGTTCTTGTGATCGACATTGGTCACCGCCGCGAGGTTTATCGCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185508 WP_011038219.1 NZ_CP066968:c1153633-1153343 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185508 NZ_CP088273:c1881014-1880781 [Phyllobacterium sp. T1293]
ATGAGCAAGCAAACGGCTATCCGCCTTCCCGATGAAATATATGAACGTCTGCAAGCCCTTTCCTCGCGCACGGGCAGGAC
AACAGCCTATTACATTCGCGAAGCTCTGGATGAATACCTGGATGATCTTGAGGATGTGTATCTGGCTGAAAAGGTACTTG
AGCGTATTCGAAGCGGTGAGGAACAAACCTATTCTCTTGAAGAAGTGAAGCAGCGTCTTGGCCTGGAGGATTGA

Structures

Antitoxin

• AlphaFold DB: Q8P668